Glucose analyzers

In addition to the method described above, there are at present, on the market, several glucose analyzers offered by several commercial companies, wherein the individual injects approximately 20 microliters of serum. This is then incubated with glucose oxidase and the peroxide or oxygen generated is measured with an oxygen sensing electrode. These instruments cannot perform the test on 1 xl nor are they designed for that purpose at the present time. 

Resin-bound enzyme was packed in Pharmacia C 10/20 jacketed columns for kinetic analysis. Glucose concentrations in hydrolyzates were determined with a YSI Model 27 glucose analyzer from Yellow Springs Instruments. 

Selected Commercial Glucose Analyzer Based on Glucose Electrode (59, 92)... 

During purification procedures cellobiase activity was monitored by measuring nitrophenol (at A42onm) release for p-nitrophenyl-/ -D-glucoside (JO). Kinetic studies and enzyme characterization were carried out using / -D-cellobiose as substrate with the product, glucose, measured with a Beckman Glucose Analyzer (JO). Assay conditions were pH 4.8 and 50°C. 

Fogt, E. J., Dodd, L. M., Jenning, E. M., and Clemens, A. H. Development and evaluation of a glucose analyzer for a glucose controlled insulin infusion system (Biostator). Clin. Chem. 24 1366-1372, 1978. 

Kim, B. S., Lee, S. C.,Lee, S. Y., Chang, H. N, Chang, Y. K., and Woo, S. 1.1994b. Production of poly(3-hydroxybutyric-co-3-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with substrate control using on-line glucose analyzer. Enz. Microbial Technol., 16, 556-561. 

The Beckman glucose analyzer Various other reactions... 

Takai, T., Kohno, M., Sakamoto, Y., Iwamoto, Y. and Matsuda, F. (1984). Clinical application of Fuji Drichem-IOOO glucose analyzer using multilayer-film elements. Hormon to Rinsho 32, 477-481. 

E510 Conrad, P.D., Sparks, J.W., Osberg, L, Abrams, L. and Hay, W.W. (1989). Clinical application of a new glucose analyzer in the neonatal intensive care unit Comparison with other methods. J. Pediatr. 114, 281-287. 

Chua KS, Tan IK. Plasma glucose measurement with the Yellow Springs Glucose Analyzer. Clin Chem 1978 24 150-2. 

Tang Z, Du X, Louie RE, Kost GJ. Effects of drugs on glucose measurements with handheld glucose meters and a portable glucose analyzer. Am J Clin Pathol 2000 113 75-86. 

Go to http //chemistry.brookscole.com/skoogfac/. From the Chapter Resources menu, choose Web Works. Locate the Chapter 29 section, and you will find links to several instrument makers who produce glucose analyzers based on enzymatic reactions. Find one company that makes a spectrophotometric analyzer and one that makes an electrochemical analyzer. Compare and contrast the features of the two instruments. 

Fig. 10. a Correlation of glucose measurement in whole blood, measured by micro-thermometric biosensor and by the Reflolux-S blood glucose analyser, b Correlation of glucose measurement in whole blood measured by micro-thermometric biosensor and by the Ektachem blood glucose analyzer... 

The first bedside artificial pancreas was introduced. The glucose analyzer allows one to control an insulin infusion system (the Biostator) [114 116. ... 

The next stage was achieved in 1967 by Updike and Hicks, who entrapped GOD in a gel of polyacrylamide, thus increasing the operational stability of the enzyme and simplifying the sensor preparation. Further investigations by Reitnauer (1972) enabled the successful application of an enzyme electrode in a prototype blood glucose analyzer. In 1975 Yellow Springs Instrument Co. (USA) commercialized a glucose analyzer (model 23 A) which was based on a patent by Clark (1970). The Lactate Analyzer LA 640 by La Roche (Switzerland) followed one year later. In this instrument the enzyme is dissolved in a buffer in a reaction chamber placed in front of the electrode. 

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