Whole blood glucose

N.J. Forrow and S.W. Bayliff, A commercial whole blood glucose biosensor with a low sensitivity to hematocrit based on an impregnated porous carbon electrode, Biosens. Bioelectron., 21 (2005) 581-587. 

The oldest example of an integrated dry reagent chemistry for quantitative analysis of a metabolite is the Dextrostix reagent strip (Miles Dia ostics) for whole blood glucose analysis. The cross section of the element is illustrated in Fig. 8a. The detection chemistry is the well-known glucose oxidase-peroxidase procedure. Approximately 50 pL of whole blood is applied to the surface of the... 

R44 Wenk, R.E., Lustgarten, J.A., Byrd, C., Patterson, D. and Hall, B. (1986). Evaluation of reagent strip reflectometry for hemoglobin and whole blood glucose. Clin. Chem. 32, 1112, Abstr. 307. 

Kost GJ, Nguyen TH, Tang Z. Whole blood glucose and lactate trilayer biosensors, drug interference, metabolism and practice guidelines. Arch Pathol Lab Med 2000 124 1128-34. 

Note Whole-blood glucose concentrations are approximately 10% to 12% lower than plasma concentrations. 

In individuals with a normal hematocrit, fasting whole-blood glucose concentration is approximately 10% to 12% lower than plasma glucose. Although the glucose concentra-... 

Application of the miniaturized biosensors for metabolite estimation in whole blood is another important concept for future development. The improvement in sensitivity, linear range and response time was achieved by miniaturization of the sensors and has been proven in the case of whole-blood glucose, urea and lactate. A useful feature of miniaturization is that the smaller the flow channel, the smaller are the dispersion and dilution effects, favouring whole blood measurement with minimum error. In the case of clinical estimations, the results of the measurement on miniaturized thermal biosensor are more reliable. 

Satoh I, Akahane M and Matsumoto K 1991 Analytical applications of immobilized acid urease for urea in flow streams Sensors Actuators B 5 241 Danielsson B 1995 Handbook of Analytical Sciences (London Academic) at press Xie B, Hedberg (Harbom) U, Mecklenburg M and Danielsson B 1993 Fast determination of whole blood glucose with a calorimetric micro-biosensor Sensors Actuators B 15-16 141-4... 

Kost GJ, Nguyen TH, and Tang Z (2000) Whole-blood glucose and lactate - trilayer biosensors, drag interference, metabolism, and practical guidelines. Archives of Pathology and Laboratory Medicine 124 1128-1134. 

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