Subcellular proteomics

Volume 43 Subcellular Proteomics Edited by Eric Bertrand and Michel Faupel... 

Jung E, Heller M, Sanchez JC, Hochstrasser DF. Proteomics meets cell biology the establishment of subcellular proteomes. Electrophoresis 2000 21 3369-3377. 

Edited by Tapas K. Kundu and Dipak Dasgupta Volume 42 Inflammation in the Pathogenesis of Chronic Diseases Edited by Randall E. Harris Volume 43 Subcellular Proteomics... 

One-step characterization of a eukaryotic cell pro-teome is difficult if not impossible to achieve. There is a growing trend in eukaryotes proteomics toward characterization of subcellular structures. The reason for this shift from global proteomics to subcellular proteomics is the complexity of eukaryotic cells and subcellular organelles. Therefore the proteomic analysis of subcellular organelles will be an important aspect of toxicoproteomics. 

Here again, not all chromatographic setups are usable for any proteomics question. The use of protein reverse phase chromatography, which has been advocated for plasma proteomics (Moritz et al. 2005), precludes in turn the use of any detergent of any type. This prevents the use of this chromatographic setup in most subcellular proteomics experiments, where detergents must be used to solubilize the membrane limiting the subcellular compartments. 

E. Bertrand andM. Faupel (eds.), Subcellular Proteomics, 185-207. 2007 Springer. 

Knowledge about the subcellular localization of a protein may provide a hint as to the function of the protein. The combination of classic biochemical fractionation techniques for the enrichment of particular subcellular structures with the large-scale identification of proteins by mass spectrometry and bioinformatics provides a powerful strategy that interfaces cell biology and proteomics, and thus is termed subcellular proteomics. ... 

Moini, M. and Huang, H., Application of capillary electrophoresis/electrospray ionization-mass spectrometry to subcellular proteomics of Escherichia coli ribosomal proteins. Electrophoresis, 25, 1981-1987, 2004. 

Brown RN, Romine ME, Schepmoes AA, Smith RD, Lipton MS. Mapping the subcellular proteome of Shewanella oneidensis MR-1 using sarkosyl-based fiaclionation and LC—MS/MS protein identification. J Proteome Res. 2010 9(9) 4454-63. doi 10.1021/prl00215h. 

Among subcellular proteomes investigated for identification of bacterial subspecies, attention was concentrated on surface and membrane-associated proteins, especially outer membrane proteins (OMPs) of Gram-negative bacteria, surface layer (S-layer) proteins of Gram-positive bacteria, flagella, and extracellular proteins (ECPs). 

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