Global investigation of small molecule interactions with proteins that constitute the cell proteome

3 Global investigation of small molecule interactions with proteins that constitute the cell (proteome) 

The interaction of small molecules with cells should also be investigated at the protein (proteome) level since the concentration (abundance), transcriptional alteration, posttranslational modification, formation of complexes with other proteins or cellular components, modulation of the activity with small molecule effectors, and compartmentation cannot be revealed at the genome level. 

Determining the exact distribution, abundance, and expression level of the proteins within different cells is very important in identifying the role of a particular protein and its interactions within the cellular signaling pathways both in healthy and diseased states. 

The human genome can be investigated at the proteome level through direct small molecule-gene product (protein) interactions using activity/affinity-based approaches. Such small molecule-protein interaction analysis serves several objectives and can be performed directly with cells, cell extracts, or alternatively applying isolated proteins in a parallel maimer. 

1 Druggability profiling of proteins using rapid affinity-based approaches 

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