Elution adsorption

DL-Leucine, resolution of, 222, 262 Leucine encephalin, 290 LFER, see Linear free energy relationships LH-releasing hormone, 263, 290 Ligate, carbon number of, 153 Ligates, solute binding to, 213 Limiting retention factors, 239 Linear elution adsorption chromatography, 58... 

Experimental and theoretical studies of the separation of 1,7- and 1,10-phenanthrolines and some substituted derivatives in linear elution adsorption chromatography,44> 145 thin-layer chromatography,146-148 and liquid-liquid partition chromatography149 have been described. 

Snyder, L. R., Linear Elution Adsorption Chromatography, Advan. 

Snyder studied the adsorption energy of 7-azaindole by means of linear elution adsorption chromatograph. It showed an adsorption energy nearly identical to that of 2-aminopyridine and a-carboline, and appeared characteristic of the — N=C— NH group. 

Snyder, L. R. Linear elution adsorption chromatography. VII. Gradient elution theory. J. Chromatogr. [Amsterdam] 13, 415 (1964). 

The scaleup considerations of column chromatography for protein isolation has been described by Charm and Matteo.l l When several hundred liters of a protein feedstream must be treated, the resin may be suspended in the solution, removed after equilibration by filtration and loaded into a column from which the desired proteins may be eluted. Adsorption onto a previously packed column was not recommended by them since they feared suspended particles would clog the interstices of the column, causing reduced flow rates and increased pressure drops across the column. The reduced flow rate may lead to loss of enzyme activity because of the increased time the protein is adsorbed on the resin. 

Large amounts of solvents with lower adsorption ability than the compounds of the sample mixture are used in the elution method. Pentane, hexane, heptane and petrol ether are frequently used by crude oil chemists as solvents for elution adsorptive analysis. 

Langmuir isotherm, 55-58 linear capacity of, 80 Linear capacity Od.i, 78-79 vs. adsorbent deactivation, 87-89 vs. adsorbent type, 89-90 vs, band width, 90 vs. K 83-87 vs. sample structure, 86 vs. sample type, 90-91 vs. solvent, 87 vs. temperature, 86-87 Linear Elution Adsorption Chromatography (LEAC), 353-354 Linear isotherm, 77-79 See also Linear capacity advantages of, 77... 

Linear elution adsorption chromatographic analysis refers to a simple technique for the repetitive, routine analysis of certain sample types for a small number of individual components. The sample is separated on a... 

Using Solvent Deasphaltening, Elution-Adsorption Chromatography, and Densimetric Characterization, Anal, Chem, (1969) 41 (4), 576-79. 

Liquid chromatography at the critical condition (LCCC) is performed at the elution-adsorption transition. It can be used to separate macromolecules with different functionalities such as chains with different chain ends or to separate linear chains from cycles. LCCC was used [77] to separate cycles from linear chains in poly(bisphenol-A-carbonate) PC. Figure 45.20 contains the LCCC trace. The trace is bimodal, with two bands, Z1 and Z2. The MALDI spectrum of Z1 displayed a large number of peaks, ranging approximately from 2.0 to 10 kDa, due to PC chains terminated with n-butyl on one side or on both sides. The MALDI spectrum of Z2 was far less crowded. It is made of cycles and one can note the systematic absence of linear chains. This implies that the LCCC separation is perfect. 

Polyakov, N.S. Larin. A.V., and Goubkina, M.L., Elution adsorption dynamics of organic substances on carbon adsorbents, Adsorpt. Sci. Technol., 10. 173-180(1994). 

The extracted metal complexes should be measured quickly after elution. Adsorption processes alter the concentrations during storage, whereas the prepared sample spot can be stored for at least 6 months Prange, 1983). 

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