Genome content reporting

The two ways genome content may be reported are in absolute amounts and in arbitrary units. When expressing genome size in absolute terms, various units may be employed. The most common unit is pico-grams (1 pg is equal to 10 12 g). Other units that may be used are daltons and nucleotide pairs. One is able to convert from one unit to another based on the following equations 1 nucleotide pair = 660 daltons, and 1 pg = 0.965 X 109 nucleotide pairs. It should be noted that these conversions are only estimates and should be considered accordingly. 

The relationship between yields, total protein concentration, and biochemical composition of proteins in plant seeds on the one hand, and the plant s genome (the effect of a species and cultivar), on the other hand, is a generally well-recognized fact (Sell et al. 2005, Triboi et al. 2000, Wieser and Seilmeier 1998). It is also well known that agronomic practice affects the content of proteins in plant seeds (Daniel and Triboi 2000, Sell et al. 2005, Triboi et al. 2000, Wieser and Seilmeier 1998). In contrast, there are not many reports on the effect of stress on plants (Daniel and Triboi 2000, 2002, Sung and Krieg 1979). 

DEF has been reported to cause extensive alterations in morphological features of erythrocyte and nuclear membranes and affected the permeability properties of rat liver mitochondrial membrane. A reduction in the activity of cytochrome-c-oxidase and NAD. H-oxidase has also been observed. Content of both DNA and RNA decreased in tissues studied within 1 month of DEF intoxication and was usually restored within 3 months. Histological study showed development of necrodystrophy in liver tissue and of fibroplastic glomerulonephritis in kidney. The deteriorating effect of DEF on cellular genome functions... 

The MIAME standard was created by the Functional Genomics Data Society, formerly known as the Microarray Gene Expression Data Society (http // www.mged.org), as an effort to provide standards to specify all the information necessary to describe and interpret unambiguously the results of a microarray experiment (74). The standard defines the contents required for compliance reports but it does not specify the format in which this data should be presented. As a consequence there are a number of different file formats for representing this data, and each public and subscription database has adopted its own format. 

Intensities are sensitive to the GC content and the DNA sequence of the reporter. Although the whole genome is known, it is still possible probes may cross-hybridise to homologous or unrelated genes. 

---