Genetic screens

A Forward Chemical Genetic Screen to Identify Inhibitors of... 

In eukaryotes, translation initiation is rate-limiting with much regulation exerted at the ribosome recruitment and ternary complex (elF2 GTP Met-tRNAjMet) formation steps. Although small molecule inhibitors have been extremely useful for chemically dissecting translation, there is a dearth of compounds available to study the initiation phase in vitro and in vivo. In this chapter, we describe reverse and forward chemical genetic screens developed to identify new inhibitors of translation. The ability to manipulate cell extracts biochemically, and to compare the activity of small molecules on translation of mRNA templates that differ in their factor requirements for ribosome recruitment, facilitates identification of the relevant target. 

Characterization of Inhibitors of Translation Identified in Chemical Genetic Screens... 

The plaintiffs are not claiming that the patent is invalid. Rather, relying on theories implicit in Moore, they seek to use informed consent liability doctrines to ensure low-cost genetic screening and testing for Canavan disease. The lawsuit is based on what I will call a breach of covenant, not a breach of promise. The term breach of covenant is appropriate, despite the contractual view in some of the literature (Schuck, 1994), because the relationship between the parties was not contractual. Breach of promise implies that their agreement was contractual in nature, which creates doctrinal problems (Palmer, 1989). 

The polymerase chain reaction (PCR), developed by Mullis, is a simple and most effective way of amplifying, i.e. producing multiple copies of, a DNA sequence. It finds applications in all sorts of areas not immediately associated with nucleic acid biochemistry, e.g. genetic screening, medical diagnostics, forensic science, and evolutionary biology. The general public is now well aware of the importance... 

Pollard SM, Yoshikawa K, Clarke ID et al (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell 4 568-580... 

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