Genes Encoding Enzymes Involved in Terpenoid Indole Alkaloid Biosynthesis

For several of the enzymes described earlier, the encoding genes have been cloned from plant species other than C. roseus. This especially holds true for enzymes involved in primary metabolism. For example, by genetic 

The expression of the Tdc gene as a function of the cell cycle and the growth of a C. roseus cell suspension culture was extensively studied by Nicoloso (285). Cells grown on the standard medium had a low, but significant, level of Tdc mRNA, after the first cell division a maximum was 

The Str gene cloned from R. serpentina has been expressed in various organisms, among others E. coli (288) and insect cells (289). In both cases, this resulted in high levels of active enzyme. Two forms were found, a high-and low-molecular-mass form both had activity, the values being similar 

A putative partial cDNA clone coding for D17H was isolated from a cDNA library of C. roseus seedlings. It has 33.3% homology at the amino 

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