Gene delivery systems nanoparticles

Pan B, Cui D, Sheng Y, Ozkan CS, Gao F, et al. (2007). Dendrimer-modified magnetic nanoparticles enhance efficiency of gene delivery system. Cancer Res. 67 8156-8163. 

Polymeric Nanoparticles or Microparticles-Based Gene Delivery System... 

Zwiorek, K., Kloeckner, J., Wagner, E., and Coester, C. (2005) Gelatin nanoparticles as a new and simple gene delivery system. Journal of Pharmacy and Pharmaceutical Science 7 22-28. 

The last talk in this session, by Andrew Lyddiatt (University of Birmingham, United Kingdom), showed how liquid-liquid extraction can address some of the problems associated with the purification of nanoparticulates (e.g., viral and nonviral gene delivery vehicles). Nanoparticles (particle size range 20-150 nm) with low diffusivity and low molalities in culture feedstocks pose unique process engineering problems in the design and implementation of selective recovery and formulation operations. This paper demonstrated how aqueous two-phase partition systems (polymer-polymer and polymer-salt) can circumvent the process bottlenecks posed by the use of... 

Chung, H. et al.. Oil components modulate physical characteristics and function of the natural oil emulsion as drug or gene delivery system, J. Contr. Rel., 71, 339, 2001. Lehrm, C. et al., Alkylcyanoacrylate drug carriers. II. Cytotoxicity of cyanoacrylate nanoparticles with different aUcyl chain lengths, Int. J. Pharm., 84, 13, 1992. 

Cationic lipids and cationic polymers are designed as gene delivery systems on the nanoscale. Especially chitosan is under focus as a biodegradable, natural biopolymer, used both as the polyplex and also as a coating material for other polyplexes. Chitosan-coated poly(isohexyl cyanoacrylate) nanoparticles have also been developed for intravenous delivery of siRNA and no evidence of toxicity was observed after intravenous administration for 30... 

Cheong, S. J., Lee, C. M., Kim, S. L. et al. 2009. Superparamagnetic iron oxide nanoparticles-loaded chitosan-linoleic acid nanoparticles as an effective hepatocyte-targeted gene delivery system. Int. J. Pharm. 372 169-176. 

FIGURE 2.1.1 Nanoparticles for drug and gene delivery system. 

It is usually difficult to add functionalities (i.e., to insert other amphiphilic molecules into a bilayer membrane) to self-assemble a bilayer nanoparticle after its self-assembly in solvents because the assembled bilayer membrane is a thermodynamically stable. If one can controlled the self-assemble phenomena, more complexed nanostrucutres can be achieved, and it will open a new stage of drug/gene delivery system. Bui et al. prepared a bilayer membrane nanoparticle via two-step self-assemblies using a solubility of amphiphilic block copolymer (Fig. 2.1.8) [108], The nanoparticles were composed of a positively charged complex core (siRNA and polyethyleneimine [PEI]) and a capsid-like (bilayer) shell. The preparation processes were divided into two steps (1) an electrostatic... 

Li Z, Zhu S, Gan K et al (2005) Poly-L-lysine-modified silica nanoparticles a potential oral gene delivery system. J Nanosci Nanotechnol 5 1199-1203... 

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