Gangliosides distribution

Negative-ion f.a.b.-m.s. has been used to complement the positive f.a.b. data in some of the aforementioned studies, and, as the sole f.a.b. procedure, has been applied to an investigation of the distribution of gangliosides in various rat-tissues, and to the analysis of synthetic lysogangliosides. ... 

Keenan, T. W., Morr6, D. J. and Huang, C. M. 1972D. Distribution of gangliosides among subcellular fractions from rat liver and bovine mammary gland. Febs. Lett. 24, 204-208. 

GDla and a sialosyltetraglycosylceramide. Recently, Levis and coworkers (9) examined the glycosphingolipids in human heart and found that human cardiac muscle contains the same gangliosides as those of human skeletal muscle. However, the distribution of gangliosides was quite different. In heart, GM3 (23%), GD3 (22%) and GM1 (16%) are the major ganlgiosides while in skeletal muscle GM3 (67%) predominates. 

Gangliosides were labelled with galactose. In the fetal cell lines (FHS) and SKCO-1 there was no marked difference between treated and untreated cells. In HT-29, SW-480, and Sw-620 cell lines, the amounts of Gm3 appeared to remain the same, but the distribution of Gm3 components was affected by butyrate. These changes, might be due to alterations in the lipid moieties or, alternatively, there might be an acetylation Of a hydroxyl group in the carbohydrate moiety, since it has been shown that the butyrate increases the amount of acetylated histones. 

T. Tai Differential distribution of gangliosides in the central nervous system J. H. van Boom Synthesis of complex oligosaccharides... 

Not only biochemical analysis, but immunohistochemical staining with antibodies against gangliosides has similarly revealed the existence and distribution of gangliosides in epidermis. Nakakuma et al. showed that epidermal keratinocytes reacted with an anti-GM3 monoclonal antibody, but not an anti-GD3 mAb.11 Expression of GM3, predominantly in the stratum corneum, was reported by Paller et al.12 In contrast, Hersey et al. detected GD2 in the basal and spinous layers of the epidermis, whereas neither GM3 nor GD3 was detected in normal skin.13 However, the epidermis adjacent to naevi and primary melanoma strongly expressed GD3.13... 

Puente, R., Garcia-Pardo, L-A., Hueso, P. 1992. Gangliosides in bovine milk Changes in content and distribution of individual ganglioside levels during lactation. Biol. Chem. Hoppe-Seyer., 373, 283-288. 

Yoshino H (1997) Distribution of gangliosides in die nervous tissues recognized by axonal form of Guillain-Bane syndrome (in Japanese). Neuroimmunology 5 174—175. 

KIO. Koenig, H., Histological distribution of brain gangliosides lysosomes as glyco-lipidoprotein granules. Naiure (London) 195, 782-784 (1962). 

Sheikh KA, Deerinck TJ, Ellisman MH, Griffin JW (1999) The distribution of ganglioside-like moieties in peripheral nerves. Brain 122 449-460. 

Koenig, H., Gaines, D., McDonald, T., Gray, R. and Scott, J. (1964) Studies of brain lysosomes. 1. Subcellular distribution of five acid hydrolases, succinate dehydrogenase and gangliosides in rat brain. J. Neurochem., 11, 729-743. 

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