Fructose-6-phosphate-2-kinase

Sabri Ml, Ederle K, Holdsworth CE, et al. 1979b. Studies on the biochemical basis of distal axonipathies. II. Specific inhibition of fructose-6-phosphate kinase by 2,5-hexanedione and methyl-butyl ketone. Neurotoxicologly 1 285-297. 

Fructose 6-phosphate kinase (phosphofructo-kinase) has attracted much attention because of its regulatory properties (Chapter n).880-882 Prokaryotic forms are somewhat simpler.883 The related fructose 6-phosphate 2-kinase is a component of a bifunctional kinase-phosphatase (Fig. 11-2, steps d and e) and has a structure similar to that of adenylate kinase.884... 

Fructose bisphosphate aldolases 699 Fructose 6-phosphate 535, 693s Fructose 6-phosphate kinase 656 Fructose-1,6-bisphosphatase 645 Fructose-2,6-bisphosphatase 646 Fruit fly. See Drosophilia melanogaster Fucose (Fuc) 165s L-Fucose isomerase 693 Fucosyltransferase 184 Fucoxanthin 22... 

A similar conclusion was arrived at by Arnold and Pette ( 2) from studies carried out on the in vitro binding of aldolase glyceraldehyde phosphate dehyrogenase, fructose-6-phosphate kinase, phosphoglycerate kinase, pyruvate kinase and lactate dehydrogenase to the structural proteins F-actin, myosin, acto-myosin and stromaprotein. 

Aerobacter aerogenes (wild-type strain PRL-R3 [2] fructose 6-phosphate kinase-deficient mutant of a uracil auxotroph of strain PRL-R3 [4]) [1, 2, 4]... 

Sapico, V. Anderson, R.L. D-Fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties. J. Biol. Chem., 244, 6280-6288 (1969)... 

The fructose 1,6-diphosphate (FDP) is the key intermediate, and the two enzymes, fructose-6-phosphate kinase and FDP aldolase, are diagnostic for this pathway. In some lactic... 

Fructose- -phosphate kinase from rabbit muscle 84,000... 

As discussed in Section 22.7, illumination of chloroplasts leads to light-driven pumping of protons into the thylakoid lumen, which causes pH changes in both the stroma and the thylakoid lumen (Figure 22.27). The stromal pH rises, typically to pH 8. Because rubisco and rubisco activase are more active at pH 8, COg fixation is activated as stromal pH rises. Fructose-1,6-bisphosphatase, ribulose-5-phosphate kinase, and glyceraldehyde-3-phosphate dehydrogenase all have alkaline pH optima. Thus, their activities increase as a result of the light-induced pH increase in the stroma. 

Answer Ribulose 5-phosphate kinase, fructose 1,6-bisphosphatase, sedoheptulose 1,7-bisphosphatase, and glyceraldehyde 3-phosphate dehydrogenase would be inhibited. All have mechanisms requiring activation by reduction of a critical disulfide bond to a pair of —SH groups. Iodoacetate reacts irreversibly with free —SH groups. 

A. triose phosphate isomerase B. fructose-1-phosphate kinase... 

D-Fructose 1-phosphate kinase catalyzes the phosphorylation of D-fructose 1-phosphate to D-fructose 1,6-bisphosphate. The enzyme has been purified from Aerobacter aerogenes326 and Bacteroides symbiosis.327... 

The molecular weight is328 75,000, and the Km for D-fructose 1-phosphate is326 0.3 mM at an optimal327 pH of 8. No phosphorylation is observed with L-ffuctose 1-phosphate, D-mannose 6-phosphate, D-fructose 6-phosphate, D-glucose 6-phosphate, or D-glucose 1-phosphate.328 The enzyme requires Mnz+ or Mg2+ for catalytic activity,328 but K+, Rb+, and NH4+ increase the enzymic activity.329 ATP inhibits the kinase, as the ratio of concentration of Mg2+ to ATP decreases below 2 1. The ATP inhibition can be released by Mg2+. Citrate (K, 0.85 mM), D-fructose 1,6-bisphosphate (K,- 4.4 mM), and D-fructose 6-phosphate (Kj 1.0 mM) inhibit D-fructose 1-phosphate kinase competitively with D-fructose 1-phosphate.328... 

D-fructose-1-phosphate kinase fructose 1-phosphate kinase kinase, 1-phosphofructo- (phosphorylating) phosphofructokinase 1... 

Anderson, R.L. Hanson, T.E. Sapico, V. D-fructose-1-phosphate kinase. Methods EnzymoL, 42C, 63-66 (1975)... 

Veiga-da-Cunha, M. Houyoux, A. Van Schaftingen, E. Overexpression and purification of fructose-1-phosphate kinase from Escherichia coli Application to the assay of fructose 1-phosphate. Protein Expr. Purif., 19, 48-52 (2000)... 

Wu LF, Reizet A, Reizer J et al. Nucleotide sequence of the Rhodobacter capsulatus fiuK gene, which encodes fructose-1-phosphate kinase Evidence for a kinase superfomily including both phos-phofructokinases of Escherichia coli. J Bacteriol 1991 173(10) 3117-3127. 

Fig. 14.2 Cellulose biosynthetic pathway in cellulose-producing acetic acid bacteria. FIP firuc-tose-1-phosphate, F6P fructose-6-phosphate, FDP fructose diphosphate, PGA phosphogluconate GHK glucose hexokinase, FHK fructose hexokinase, IPFK fructose-1-phosphate kinase, FBP fructose blsphosphatase, PGI phosphoglucose isomerase, PGM phosphoglucomutase, UGP UDP-glucose pyrophosphorylase, G6PD glucose-6-phosphate dehydrogenase, PTS phosphotransferase system EMP Embden-Myerhoff pathway...

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