D-Fructose 1-phosphate kinase

D-Fructose 1-phosphate kinase catalyzes the phosphorylation of D-fructose 1-phosphate to D-fructose 1,6-bisphosphate. The enzyme has been purified from Aerobacter aerogenes326 and Bacteroides symbiosis.327... 

The molecular weight is328 75,000, and the Km for D-fructose 1-phosphate is326 0.3 mM at an optimal327 pH of 8. No phosphorylation is observed with L-ffuctose 1-phosphate, D-mannose 6-phosphate, D-fructose 6-phosphate, D-glucose 6-phosphate, or D-glucose 1-phosphate.328 The enzyme requires Mnz+ or Mg2+ for catalytic activity,328 but K+, Rb+, and NH4+ increase the enzymic activity.329 ATP inhibits the kinase, as the ratio of concentration of Mg2+ to ATP decreases below 2 1. The ATP inhibition can be released by Mg2+. Citrate (K, 0.85 mM), D-fructose 1,6-bisphosphate (K,- 4.4 mM), and D-fructose 6-phosphate (Kj 1.0 mM) inhibit D-fructose 1-phosphate kinase competitively with D-fructose 1-phosphate.328... 

D-fructose-1-phosphate kinase fructose 1-phosphate kinase kinase, 1-phosphofructo- (phosphorylating) phosphofructokinase 1... 

Sapico, V. Anderson, R.L. D-Fructose 1-phosphate kinase and D-fructose 6-phosphate kinase from Aerobacter aerogenes. A comparative study of regulatory properties. J. Biol. Chem., 244, 6280-6288 (1969)... 

Anderson, R.L. Hanson, T.E. Sapico, V. D-fructose-1-phosphate kinase. Methods EnzymoL, 42C, 63-66 (1975)... 

This enzyme [EC 2.7.1.11], also known as phosphohexo-kinase and phosphofructokinase 1, catalyzes the reaction of ATP with D-fructose 6-phosphate to produce ADP and D-fructose 1,6-bisphosphate. Both D-tagatose 6-phosphate and sedoheptulose 7-phosphate can act as the sugar substrate. UTP, CTP, GTP, and ITP all can act as the nucleotide substrate. This enzyme is distinct from that of 6-phosphofructo-2-kinase. See also ATP GTP Depletion... 

PPi-PFK <1, 30, 35, 37-39, 41, 44> [31-33, 35, 36, 38-40, 44, 47] PPi-dependent phosphofructokinase <1, 35, 37, 39, 40> [33, 35, 39-41] diphosphate-D-fructose-6-phosphate 1 -phosphotransferase fructose 1,6-bisphosphatase <42> [45] inorganic pyrophosphate-dependent phosphofructokinase inorganic pyrophosphate-phosphofructokinase phosphotransferase, pyrophosphate-D-fructose 6-phosphate 1-pyrophosphate D-fructose-6-phosphate 1-phosphotransferase pyrophosphate-D-fructose 6-phosphate 1-phosphotransferase pyrophosphate-D-fructose 6-phosphate 1-phosphotransferase pyrophosphate-D-fructose 6-phosphate phosphotransferase pyrophosphate-dependent phosphofructo-1 -kinase pyrophosphate-dependent phosphofructokinase... 

Fig. 15.2. Pathways of fructose metabolism. 15.4, Fructose-1-phosphate aldolase 15.5, fructose-1,6-diphosphatase 15.7, D-glycerate kinase. F-l-P, Fructose-1-phosphate F-6-P, fructose-6-phosphate F-l,6-DiP, fructose-1,6-diphosphate Glc-6-F, glucose-6-phosphate ATP, adenosine triphosphate ADP, adenosine diphosphate...

Hexokinase catalysed syntheses of 6-amino-6-deoxy-D-glucose and -D-gluconate 6-phosphates, 6-amino-6-deoxy-D-fructose 6-phosphate and 1,6-bisphosphate, and 5-amino-5-deoxy-D-ribulose 5-phosphate have been reported. From a study of the properties and reactivity in enzymic reactions of these compounds, it was concluded that they were excellent isosteric analogues of the normal metabolic intermediates except for reactions catalysed by kinases. A preparative enzymic synthesis of uridine 5 -diphospho-2-acetamido-2-deoxy-D-glucose used chemically synthesized 2-acetamido-2-deoxy-D-glucose 1-phosphate and uridine triphosphate as reactants, and a calf liver enzyme extract containing uridine-5 -phospho-A-acetylglucosamine pyrophosphorylase. ... 

The i C-n. m.r. spectrum of 1,2-O-ethylene-a-D-glucofuranose (2) has been recorded and assigned, and detailed 1- and 2-Dn.m.r. experiments supplemented by molecular mechanics calculations allowed the unequivocal assignment of all C-, and P-n.m.r. resonances of o-fructose 2,6-diphosphate. The study showed, in addition, that the activator of fiructose 6-phosphate 1-kinase is the -anomer in the 3 conformation. ... 

---