Fractionation by high-performance

CALDWELL c R (2001) Oxygen radical absorbance capacity of the phenolic compounds in plant extracts fractionated by high-performance liquid chromatography, ,4Biochem, 293, 232-8. 

Separation of green tea catechins fraction by high-performance/pressure liquid chromatography. 

Redden, RR. and Huang, Y.-S. 1991. Automated separation and quantitation of lipid fractions by high-performance liquid chromatography and mass detection. J. Chromatogr. 567 21-27. 

G4. Gallice, P., Fournier, N., Crevat, A., et al., Separation of one uremic middle molecular fraction by high performance liquid chromatography. Kidney Int. 23, 764-766 (1983). 

Brumley, W.C., C.M. Brownrigg, and A.H. Grange. 1993. Determination of toxaphene in soil by electron-capture negative ion mass spectrometry after fractionation by high-performance gel permeation chromatography. J. Chromatogr. 633 177-183. 

There are many traditional methods for isolation of chitin oligomers. The procedures involved acid hydrolysis, neutralization, demineralization, fractionation by charcoal-celite column, fractionation by high-performance liquid chromatography (HPLC), and lyophilization. 

The concept that molecular weight and polarity play a role in determining the composition of an asphaltene fraction is very realistic but does not present any indication of the nature of the polynuclear aromatic systems in the asphaltene fraction of petroleum. Thus, the purpose of this chapter is to present new data that provide indications of the ring-size distribution in petroleum asphaltenes. The evidence is accumulated by asphaltene fractionation and subsequent examination of the fractions by high-performance liquid chromatography (HPLC) using a UV detector. 

D. Dupuy and S. Szabo, Measurement of tissue sulfhydryls and disulfides in tissue protein and non-protein fractions by high-performance liquid-chromatography using electrochemical detection, J. Liq. Chromatogr., 1987, 10, 107-119. 

Bjerrum, OW, Nielsen, H and Borregaard, N (1989) Quantitative analysis of phosphoUpids and demonstration of plasmalogen in human neutrophil subcellular fractions by high-performance liquid chromatography. Scand J Clin Lab Invest, 49, 613-622. 

Styrene-butadiene copolymers have been fractionated by high performance liquid chrom-atography. ... 

The total phosphoms content of the sample is determined by method AOCS Ja 5-55. Analysis of phosphoUpid in lecithin concentrates (AOCS Ja 7-86) is performed by fractionation with two-dimensional thin-layer chromatography (tic) followed by acid digestion and reaction with molybdate to measure total phosphorous for each fraction at 310 nm. It is a semiquantitative method for PC, PE, PI, PA, LPC, and LPE. Method AOCS Ja 7b-91 is for the direct deterrnination of single phosphoHpids PE, PA, PI, PC in lecithin by high performance Hquid chromatography (hplc). The method is appHcable to oil-containing lecithins, deoiled lecithins, lecithin fractions, but not appHcable to lyso-PC and lyso-PE. 

Boss, H. J., Rohde, M. F., and Rush, R. S., Multiple sequential fraction collection of peptides and glycopeptides by high-performance capillary electrophoresis, Anal. Biochem., 230, 123, 1995. 

Yim, K. W., Fractionation of the human recombinant tissue plasminogen activator (rtPA) glycoforms by high-performance capillary zone electrophoresis and capillary isoelectric focusing, /. Chromatogr , 559, 401, 1991. 

Dai, Y. Li, L. Roser, D. C. Long, S. R. Detection and identification of low-mass peptides and proteins from solvent suspensions of Escherichia coli by high performance liquid chromatography fractionation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Comm. Mass Spectrom. 1999,13,73-78. 

The methylene blue reaction can also be used in a fractionation procedure for surfactants. The complexes with methylene blue can be collected in an organic solvent, concentrated, dissolved in methanol, and separated by high-performance liquid chromatography [205]. A variation of this method, permitting the collection of surfactant from large volumes of sample, should be workable in seawater. 

A more traditional but still successful method for the detection of a protein phosphorylation is by radioactive labeling with 35P. The labeled protein is digested, the peptides are separated by high-performance liquid chromatography, and the phosphorylated peptides are detected in specific fractions via their radioactivity. The fraction with the phosphorylated peptides can be further analyzed by mass spectrometry (Figeys et al., 1999). 

Schulten, H.-R., and Soldati, F. (1981). Identification of ginsenosides from Panax ginseng in fractions obtained by high-performance liquid chromatography by field desorption mass spectrometry, multiple internal reflection infrared spectroscopy and thin layer chromatography. J. Chromatogr. 212, 37-i9. 

Tong, H. Y., and F. W. Karasek, Quantitation of Polycyclic Aromatic Hydrocarbons in Diesel Exhaust Particulate Matter by High-Performance Liquid Chromatography Fractionation and High-Resolution Gas Chromatography, Anal. Chem., 56, 2129-2134(1984). 

Seiber JN, Glotfelty DW, Lucas AD, et al. 1990. A multiresidue method by high performance liquid chromatography-based fractionation and gas chromatographic determination of trace levels of pesticides in air and water. Arch Environ Contam Toxicol 19 583-592. 

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