Fourier microspectroscopy

Kansiz, M. Heraud, P. Wood, B. Burden, F. Beardall, J. McNaughton, D. Fourier transform infrared microspectroscopy and chemometrics as a tool for the discrimination of cyanobacterial strains. Phytochemistry 1999,52,407-417. 

Mouille, G., Robin S., Lecomte, M., Pagant S., and Hofte, H., 2003, Classification and identification of Arabidopsis cell wall mutants using Fourier-Transform InfraRed (FT-IR) microspectroscopy, Plant J. 35 393-404. 

Fourier transform infrared microspectroscopy (FTIR) and Raman microspectroscopy provide quantitative information about the chemical microstructure of heterogeneous solid foods (Cremer and Kaletunq, 2003 Piot et al., 2000 Thygesen et al., 2003) without sample destruction. 

Fourier transform infrared microspectroscopy couples both interferometry and microscopy into an integrated instmment. Since interferometry is an important... 

Aparicio, S., Doty, S. B., Camacho, N. P. et al. (2002) Optimal methods for processing mineralized tissues for Fourier transform infrared microspectroscopy. Calcif. Tissue Int. 70 422-9. 

Fourier transform infrared microspectroscopy applied to Byzantine manuscripts in the Special Collections Department of the University of Chicago Library revealed the use of numerous additives to the paint mixture including kaolin, hide glue, egg yolk, and other proteinaceous materials. Some evidence suggests that cochineal was used as a red pigment. 

Another objective of this chapter is to introduce the use of Fourier transform infrared (FT-IR) microspectroscopy for the analysis of pigments and to provide some background about the technique. 

As described in the following sections, IR and Raman spectroscopy, using modem Fourier transform techniques such as IR microspectroscopy, offer excellent analytical tools for the burgeoning field of combinatorial chemistry. 

Biomolecular investigation of human substantia nigra in Parkinson s disease by synchrotron radiation Fourier transform infrared microspectroscopy. Arch. Biochem. Biophys., 459, 241-8. 

Levine, S.M., Wetzel, D.L. and Eilert, A.J. (1994) Neuropathology of twitcher mice examination of histochemistry, immunohistochemistry, lectin histochemistry and Fourier transform infrared microspectroscopy. Int.J. Dev. Neurosci., 12, 275-88. 

Wetzel, D.L and Williams, G.P. (1998) Localized (5 mm) probing and detailed mapping of hair with synchrotron powered FT-IR microspectroscopy, in Proceedings, nth International Conference on Fourier Transform Spectroscopy, Athens, Georgia, August 1997 (eds J.A. deHaseth and R.A. Dluhy), American Institute of Physics, Woodbury, New York. 

N.S. (2005) Synchrotron Fourier transform infrared microspectroscopy a new tool to monitor the fate of organic contaminants in plants. Microchem. J.,... 

Fourier transform infrared microspectroscopy detects changes in protein secondary structure associated with desiccahon tolerance in developing maize embryos. Plant Physiol, 116, 1169-77. 

Wetzel, D.L. and LeVine, S.M. (1998) Fourier transform infrared (FT-IR) microspectroscopy a new molecular dimension for tissue or cellular imaging dimension for tissue or cellular imaging and in situ chemical analysis. Cell. Mol Biol, 44 (1), 1-280. 

R., Belton, P. and Roberts, K. (1992) Fourier transform infrared microspectroscopy is a new way to look at plant cell walls. Plant Physiol, 100,1940-7. 

Raman microspectroscopy is the fastest and most powerful tool for analysis of phase transformations in contact loading. It can additionally provide information on residual stresses and/or chemical changes in the surface layers. However, limited databases of Raman spectra and difficulties with the interpretation of Raman spectra, as well as low accuracy of existing predictive tools for calculations of Raman spectra of solids, make it necessary to complement Raman data with electron or X-ray diffraction studies. Fourier-transform infrared microspectroscopy is another technique that can provide useful information on structural and compositional changes in the surface layer. 

Fourier transform infrared microspectroscopy is applied for the characterisation of a coated interface and a polymer blend. The coated sample investigated is prepared by coating a urethane paint on an ethylene-ethyl acrylate copolymer whose ethyl-ester group is partially hydrolysed (EAA/EEA). It is demonstrated that a mixed phase is formed along the coated interface between the paint and the EAA/EEA. Interaction between molecules of the urethane paint and the EAA/EEA is observed. For a polymer blend prepared by mixing PP and polycarbonate. 

Figure 6 A series of spectra (right) from the various layers of a pigmented rat retina (left). Spectra were collected from photoreceptor outer segments (OS, spectrum 1), inner segments (IS, spectrum 2), outer nuclear layer (ONL, spectrum 3), outer plexiform layer (OPL, spectrum 4), inner nuclear layer (INL, spectrum 5), and inner plexiform layer (IPL, spectrum 6). Note the elevated absorbance values for 0= C-H (B), CH2 (C) NH (A), 0=0 (D) in the outer segments and the elevated absorbance values for P = 0 (E) and H-C-OH (F) in the outer nuclear layer. The CH2 NH ratio is also large in the inner plexiform layer. (Reprinted with permission from LeVine SM, Radel JD, Sweat JA, and Wetzel DL (1999) Microchemical analysis of retina layers in pigmented and albino rats by Fourier transform infrared microspectroscopy. Biochimica et Biophysica Acta-General Subjects 1473 409-417 Elsevier.)...

---