FLUOROSCEIN-5-ISOTHIOCYANATE FITC

CA Index Name Spiro[isobenzofuran-l(3/7),9 -[9//] xanthen] -3-one, 3, 6 -dihydroxy-5-isothiocyanato- 

Other Names Fluorescein, 5-isothiocyanato- 5-Isothiocyanatofluorescein FITC isomer I Fite isomer 1 Fluorescein 5-isothiocyanate Fluorescein isothiocyanate isomer 1 Fluorescein isothiocyanate isomer I Fluoroscein-5-isothiocyanate Merck Index Number 4120 Chemical/Dye Class Xanthene Molecular Formula C21H11NO5S Molecular Weight 389.38 

Physical Form Orange-yellow crystal Orange solid Solubility Soiubie in acetone,, A-dimethyiformamide, dimethyi suifoxide, ethanoi, methanol Melting Point 193 °C (decompose) 160 C (decompose)  

Imaging/Labeling Applications Amino groups antihodies hiotin cells 48 cellulose 

Industrial Applications Gel coating/gel monolith for fiber-optic sensor applications ultrathin films  

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Fluoroscein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) are commercially available from, for example, Sigma Chemical Co. (Appendix 3). 

Most compounds of interest for nasal delivery have a molecular weight in excess of 1,000 Da and until recently were thought to cross the cells endocytically. However, a recent study in rats has shown the transport of fluoroscein isothiocyanate (FITC)-labeled dextran (M. Wt. = 3,000 Da) to be via the paracellular pathway, with only a proportion moving endocytically. Hardly any transport of FITC-labeled dextran with a molecular weight of 10,000 Da was observed unless a penetration enhancer was coadministered, but the penetration enhancer, sodium taurodihydrofusidate (STDHF), caused cell swelling and extrusion of mucus. 

An avidin-biotin system has been used to attach antibodies in the bilayer of DDSs. Xiao et al. developed a three-step strategy to improve the tumor-to-tissue ratio of anticancer agents [184], Two antibodies specific for the CA-125 antigen that is highly expressed on NIH OVCAR-3 cells were used. These cells were prelabeled with biotinylated anti-CA-125 antibody and fluoroscein isothiocyanate (FITC)-labeled streptavidin (SAv) prior to administration of biotinylated liposomes. Both antibodies were specifically bound to the cell surface of OVCAR-3 cells but not to SK-OV-3 cells, which do not express the specific antibody. Antibody biotinylation did not affect its immunoreactivity. 

FBS FGF FITC GO-phase G1-phase G2-phase GMP, GDP, GTP foetal bovine serum fibroblast growth factor fluoroscein isothiocyanate the resting stage of the cell cycle the first gap in the cell cycle (between M and S) the second gap in the cell cycle (between S and M) guanosine monophosphate, diphosphate, triphosphate... 

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