Fluorescein isothiocyanate isomer

Fluorescein isothiocyanate isomer 1 (FITC), which provides good sensitivity for primary and secondary amines, was chosen as a reagent for derivatization of dimethylamine (DMA) and other... 

Other Names Fluorescein, 5-isothiocyanato- 5-Isothiocyanatofluorescein FITC isomer I Fluorescein 5-isothiocyanate Fluorescein isothiocyanate isomer 1 Fluorescein isothiocyanate isomer I Fluoroscein-5-isothiocyanate... 

Fluorescein isothiocyanate Isomer I (5-isocyanato Isomer) [3326-32-7] [27072-45-3 mixture... 

Acetylcholineesterase A 350 pM diameter coherent imaging fiber coated on the distal surface with a planar layer of analyte-sensitive polymer that was thin enough not to affect the fiber s imaging capabilities. It was applied to a pH sensor array and an ACh biosensor array (each contain 6000 optical sensor). Fibers were coated with an immobilized layer of poly (hydroxyethylmethacrylate)-N-flurosceinylacrylamide and AChE-fluorescein isothiocyanate isomer poly (acryloamide-co-N-acryl oxysuccinimide), respectively. The response time of the pH sensor was 2 s for a 0.5 unit increase in pH. The biosensor had a detection limit of 35 pM ACh and a linear response in the range 0.1 mM. [90]... 

Fluorescein isothiocyanate, isomer I (FITC) Carbonate-bicarbonate buffer, 0.1 M, pH 9.0 Immunoglobulin preparation (10 mg/ml in saline) Phosphate-buffered saline, pH 7.5 (PBS) Sephadex G-50 medium... 

Muramoto, K. Kamiya, Ff. Kawauchi, H. Meguro, H. High performance liquid chromatography of fluorescein isothiocyanate isomers and intermediate products. Anal. Sci. 1985,1, 447 50. 

Steinbach, G. Characterization of fluorescein isothiocyanate. Synthesis and testing methods for fluorescein isothiocyanate isomers. Acta Histochem. 1974, 50, 19-34. 

Nitrofluorescein is prepared by the method of Coons and Kaplan [220] from resorcinol and 4-nitrophthalic acid. The two isomers formed are separated by fractional crystallization. The nitro group is reduced as described by McKinney el al. [221]. Finally, fluorescein isothiocyanate is obtained from aminofluorescein by treatment with thiophosgene [222]. 

FITC is probably the most popular fluorescent probe ever created. An isothiocyanate derivative of fluorescein is synthesized by modification of its lower ring at the 5- or 6-carbon positions. The two resulting isomers are nearly identical in their reactivity and spectral properties, including excitation and emission wavelengths and intensities. Their chemical differences, however, may affect the separation of modified proteins from excess reagent or the analysis of tagged molecules by electrophoresis. For this reason, most manufacturers purify the carbon-5 derivative as the FITC reagent of choice. 

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