Fluorescence resonant energy transfer FRET

Toth PT, Ren D, Miller RJ (2004) Regulation of CXCR4 receptor dimerization by the chemokine SDF-lalpha and the HIV-1 coat protein gpl20 a fluorescence resonance energy transfer (FRET) study. J Pharmacol Exp Ther 310 8-17 Tran PB, Miller RJ (2005) HIV-1, chemokines and neurogenesis. Neurotox Res 8 149-158 Tran PB, Ren D, Veldhouse TJ, Miller RJ (2004) Chemokine receptors are expressed widely by embryonic and adult neural progenitor cells. J Neurosci Res 76 20-34... 

When the proteins are in close proximity the Europium-cryptate emission can be absorbed by the acceptor (such as allophycocyanin [APC], or XL) which emits at a higher wavelength. When the two proteins are far apart, no fluorescence resonance energy transfer (FRET) occurs. 

Toth PT, Ren D, Miller RJ. Regulation of CXCR4 receptor dimerization by the chemokine SDF-lalpha and the HIV-1 coat protein gpl20 a fluorescence resonance energy transfer (FRET) study. J Pharmacol Exp Ther 2004 310(1) 8-17. 

Following previous work, Hagiwara and collaborators [71] recently prepared 5 -terminal acridone-labeled DNAs, using the succinimidyl ester 24 of the acridone acetic acid 23 reported before [69], and evaluated their use as donors for a fluorescence resonance energy transfer (FRET) system in combination with 3 -dabcyl-tagged DNA... 

In an important extension of the above strategy, fluorescence resonance energy transfer (FRET) was used to screen room-temperature Heck reactions.41 FRET had previously been... 

Didenko, V. V. (2001). DNA probes using fluorescence resonance energy transfer (FRET) Designs and applications. Biotechniques 31,1106-1116. 

The term filterFRET here refers to intensity-based methods for calculating fluorescence resonance energy transfer (FRET) from sets of images of the preparation collected at different excitation and/or emission wavelength. The term is not intended to imply that interference filters are actually present in the setup very similar considerations apply when donor- and acceptor fluorophores are spectrally resolved by other means, such as monochromators or spectral detectors. 

Ecker, R. C., de Martin, R., Steiner, G. E. and Schmid, J. A. (2004). Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry A 59, 172-81. 

Gu, Y., Di, W. L., Kelsell, D. P. and Zicha, D. (2004). Quantitative fluorescence resonance energy transfer (FRET) measurement with acceptor photobleaching and spectral unmixing. J. Microsc. 215, 162-73. 

Van Munster, E. B., Kremers, G. J., Adjobo-Hermans, M. J. and Gadella, T. W., Jr. (2005). Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching. J. Microsc. 218, 253-62. 

Fluorescence resonance energy transfer (FRET) has also been used very often to design optical sensors. In this case, the sensitive layer contains the fluorophore and an analyte-sensitive dye, the absorption band of which overlaps significantly with the emission of the former. Reversible interaction of the absorber with the analyte species (e.g. the sample acidity, chloride, cations, anions,...) leads to a variation of the absorption band so that the efficiency of energy transfer from the fluorophore changes36 In this way, both emission intensity- and lifetime-based sensors may be fabricated. 

An increase in sensitivity and reliability of chip analysis can also be achieved by using fluorescence resonance energy transfer (FRET). For this purpose both the probe and the target are labeled with a fluorophor. When the emission spectrum of the donor, e.g. Cy5, overlaps with the absorption spectrum of the acceptor, e.g. Cy5.5, and the donor and the acceptor are at a certain distance from each other, energy is transferred from the donor to the acceptor on excitation of the donor fluorophor. 

The already critical need for molecular-scale compositional mapping will increase as more complex structures are assembled. Currently, electron microscopy, scanning probe microscopy (SPM) and fluorescence resonance energy transfer (FRET) are the only methods that routinely provide nanometer resolution. 

Fluorescence quantum yields, 20 512-513 Fluorescence resonance energy transfer (FRET), 17 636 26 804 Fluorescence spectra, of polymethine dyes, 20 512-513... 

Wang X, Guo X (2009) Ultrasensitive Pb2+ detection based on fluorescence resonance energy transfer (FRET) between quantum dots and gold nanoparticles. Analyst 134 1348-1354... 

Sekar RB, Periasamy A (2003) Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J Cell Biol 160 629-33... 

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