FRET fluorescence resonance

CPM central pontine myelinolysis FRET fluorescence resonant energy transfer... 

FRET (fluorescence resonance energy transfer) Several Proximity event measured by radiationless energy transfer between donor and acceptor fluorophores followed by photon emission by the latter... 

Abbreviations AOD, Acousto-optical deflection BCB, bisbenzyocyclobutadiene CCD, indirect contact conductivity detection CL, chemiluminescence ECD, electron capture detector FCS, fluorescence correlation spectroscopy FRET, fluorescence resonance energy transfer ICCD, integrated contact conductivity detection GMR, giant magnetoresistive LED-CFD, light emitting diode confocal fluorescence detector LIF, laser-induced fluorescence LOD, limit of detection MALDI, matrix-assisted laser desorption ionization PDMS, poly(dimethylsiloxane) PMMA, poly(methylmetha-crylate) SPR, surface plasmon resonance SVD, sinusoidal voltammetric detection TLS, thermal lens spectroscopy. 

More recently double stranded DNA-binding dyes, (e.g., SYBR Green), have been introduced (Giulietti et al. 2001) which removed the need for an expensive, specific probe to be designed. Other sophisticated tools have been developed to work in conjunction with the Taqman method, for example molecular beacons, scorpions and hybridisation probes. These techniques rely on the FRET (Fluorescence Resonance Energy Transfer) principle but do not require the nuclease activity of the Taq polymerase. The different real-time... 

FIGURE 4.4 Assays commonly used in ion channel research. FRET = fluorescence resonance energy transfer Im = transmembrane current Vm = transmembrane potential AVm = change in transmembrane potential A mn = change in concentration of intracellular ion AAS = atomic absorption spectrometry. 

A = agar plate-based assay S = solution-based assay FRET = fluorescence resonance energy transfer FP = fluorescence polarization FCS = fluorescence correlation spectroscopy... 

CD = circular dichroism DCPIP = 2, 6-dichloro-phenolindophenol EPR = electron paramagnetic resonance ESI-MS = electrospray ionization mass spectrometry FTIR = Fourier transform infrared FRET = fluorescence resonant energy transfer 5 -GMP = guanosine-5 -monopho-sphate L-Met = L-methionine NAAD = nicotinic acid adenine dinucleotide MS = mass spectrometry NMR = nuclear magnetic resonance PEEK = polyetheretherketone SF = stopped-flow SVD = singular value decomposition tu = thiourea. 

FRET fluorescence resonance energy transfer PDMS polydimethylsiloxane TIR total internal reflection... 

FRET Fluorescence Resonance Energy Transfer, or Forster Resonance... 

Abbreviations Acs, acetyl coenzyme A synthetase CheA P, phosphorylated CheA CheY P, phosphorylated CheY FRET, fluorescence resonance energy transfer GFP, green-fluorescent protein PTS, phosphoenolpyruvate-dependent carbohydrate phosphotransferase system YFP, yellow-fluorescent protein. 

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