Spectral imaging microscopy

As mentioned above, spectral imaging microscopy is a form of multidimensional fluorescent microscopy where a fluorescent emission spectrum is acquired at each coordinate location in the sample. This mode of imaging has been implemented for wide field, confocal, and two-photon laser scanning microscopy, and several excellent... 

Ecker, R. C., de Martin, R., Steiner, G. E. and Schmid, J. A. (2004). Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry A 59, 172-81. 

Fluorescence spectral imaging microscopy (FSPIM)—a method that uses a spectroscopic approach to quantify changes in the acceptor intensity at donor excitation wavelengths [24, 27, 51, 52] (see Chapter 8) ... 

Renishaw, PLC. European Patent 0 543 578 A1 (filed Nov. 13, 1992 published May 26, 1993). MD Schaeberle, JF Turner II, PJ Treado. Multiplexed acousto-optic tunable filter (AOTF) spectral imaging microscopy. Proc SPIE 2713, 1994. 

Vereb, G., Jares-Erijman, E., Selvin, P. R. and Jovin, T. M. (1998). Temporally and spectrally resolved imaging microscopy of lanthanide chelates. Biophys. J. 74, 2210-22. 

Hanley, Q. S., Arndt-Jovin, D. J. and Jovin, T. M. (2002). Spectrally resolved fluorescence lifetime imaging microscopy. Appl. Spectrosc. 56,155-66. 

Multi-spectral imaging and linear unmixing add a whole new dimension to laser scanning fluorescence microscopy. Biotechniques 31, 1272-8. 

Spectral imaging fluorescence microscopy. Genes Cells 7, 881-7. 

Zimmermann, T., Rietdorf, J. and Pepperkok, R. (2003). Spectral imaging and its applications in live cell microscopy. FEBS Lett. 546, 87-92. 

Zimmermann, T. (2005). Spectral imaging and linear unmixing in light microscopy. Adv. Biochem. Eng. Biotechnol. 95, 245-65. 

Zucker, R. M., Rigby, P., Clements, I., Salmon, W. and Chua, M. (2007). Reliability of confocal microscopy spectral imaging systems Use of multispectral beads. Cytometry A 71, 174—89. 

Tinnefeld, R, Herten, D.P., and Sauer, M. 2001. Photophysical dynamics of single molecules studied by spectrally-resolved fluorescence hfetime imaging microscopy (SFLIM). J. Phys. Chem. A 105 7989. 

Dickinson, M. E., Bearman, G., Tille, S., Lansford, R., and Eraser, S. E. 2001. Multi-spectral imaging and hnear unmixing add a whole new dimension to laser scaiming fluorescence microscopy. Biotechniques 31(6) 1272-8. 

ADMP AES AFM ATP AXSIA Atom-centered Density Matrix Propagation Atomic Emission Spectroscopy Atomic Force Microscopy Adenosine 5 -Triphosphate Expert Spectral Image Analysis... 

B. Rajwa, T. Bemas, H. Acker, J. Dobracki and J.P. Robinson, Single- and two-photon spectral imaging of intrinsic fluorescence of transformed human hepatocytes, Microscopy Research and Technique, 70(10), 869-879 (2007). 

Confocal microscopy already allows the mapping of slices of a sample by spectral imaging at different depths. By combining traditional spectroscopy with microscopic and macroscopic imaging capabilities, complex heterogeneous samples can be analysed. In summary, the technique allows both the physical characteristics e.g. particle size and distribution, and chemical characteristics, e.g. the structural properties, of a sample to be investigated simultaneously. 

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