Fluorescein, FITC

Abbreviations EPR, electron paramagnetic resonance FITC, fluorescein-5 -isothiocyanate lAEDANS, iV-iodoacetyl-N -(5-sulfo-l-naphthyl)ethylenediamine NCD, fluorescent yV-cyclohexyl-N -(4-dimethyl-amino-a-naphthyl)carbodiimide RITC, rhodamine-5 -isothiocyanate DPPE, dipalmitoylphosphatidyl-ethanolamine PE, egg phosphatidyl-ethanolamine ANS, 8-anilino-l-naphthalene sulfonate DPH, diphenylhexatriene e-ADP, l,iV -ethanoadenosine-5 -diphosphate TNP-ADP, 2 [3 ]-0-(2,4,6-trinitrophe-nyl)adenosine-5 -diphosphate. 

Fe-TPAA Fe(III)-tris[N-(2-pyridylmethyl)-2-aminoethyl] amine Fe-TPEN Fe(II)-tetrakis-N,N,N, N -(2-pyridyl methyl-2-aminoethyl)amine FFA Free fatty acids FGF Fibroblast growth factor FID Flame ionization detector FITC Fluorescein isothiocyanate FKBP FK506-binding protein FLAP 5-lipoxygenase-activating protein... 

IPHC, Intraperitoneal hyperthermic chemoperfusion/chemotherapy MMC, Mitomycin C IP, Intraperitoneal SOD, Superoxide dismutase Nd YAG, Neodymium-doped yttrium aluminium garnet Nd Y3A15012 NIR, Near infrared FITC, Fluorescein isothiocyanate PEG, Polyethylene glycol FA, Fohc acid CDDP, Cisplatin TEM, Transmission electron microscopy... 

List of Abbreviations BCIP, 5-bromo-4-chloro-3-indolyl phosphate, toluidine salt CCD, charged-coupled device CNS, central nervous system cDNA, copy or complimentary RNA cRNA, copy or complimentary RNA DNase, deoxyribonuclease FITC, fluorescein isothiocyanate mRNA, messenger RNA NBT, nitro blue tetrazolium choride PCR, polymerase chain reaction RNase, ribonclease... 

Abbreviations AMCA, 7-amino-4-methylcoumarin B-PE, B phycoerythrin Cy, cyanine DAMC, diethylaminocoumarin FITC, fluorescein isothiocyanate RB-200-SC, lissamine rhodamine sulfonylchloride R-PE, R phycoerythrin SITS, 4-acetamido-4 -isothiocyanato-stilbene-2,2 -disulfonic acid TRITC, tetramethyl rhodamine isothiocyanate XRITC, rhodamine X isothiocyanate. Information obtained from refs. 2, 9, and 10. 

Other nucleotides, such as dCTP, can be substituted for dATP at equal concentrations. The addition of unlabeled dNTPs to the reaction allows for a longer tail to be added by TdT without the problem of steric hindrance caused by the modification on the nucleotide (biotin, DIG, or FITC). Fluorescein-labeled nucleotides should be protected from light at all times to avoid loss of signal. 

MAA Methacrylic acid, EGDMA Ethylenglycoldimethacrylate, DAM Diethylaminoethylaminoethylmethacrylate, VPY4-Vinylpiridine DVB Divinylbenzene, CMMC 7-Carboxymethoxy-4-methylcoumarin, AA Allylamine, 4MU 4-Methylumbelliferone, OP A o-Phthalic dialdehyde + beta-mercaptoethanol, APBA 3-Aminophenylboronic acid, TBA 3-Thiopheneboronic acid, DTAF 5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein, FITC Fluorescein isothiocyanate... 

SpA, Staphylococcal protein A PpL, protein L from Peptostreptococcus Magnus IgG, immunoglobulin G Gla, y-carboxyglutamic acid SPR, surface plasmon resonance ELISA, enzyme-linked immunosorbent assay FITC, fluorescein isothiocyanate NMR, nuclear magnetic resonance... 

Fig. 2 Membrane topography of the a-LTX pore. Cross-section of the a-LTX tetramer embedded in a membrane (as observed in liposomes) (Orlova et al. 2000) is shown alongside the cut-open voltage-dependent K+ channel (Kvl.2) (Long et al. 2005) and Ca2+ release channel (ryanodine receptor) (Serysheva et al. 2005). Fully hydrated cations and molecules known to permeate through the respective channels are shown next to each reconstruction (FITC, fluoresceine isothiocyanate NE, norepinephrine). The narrowest part of the a-LTX channel is 10 A. Molecular images were produced using the UCSF Chimera package (Pettersen et al. 2004).

Antiserum at 37°, 15 min Wash 0°. +Lectin 100-300 yg/ml at 0°, 30 min Wash, 0° Rabbit anti-lectin serum, 0°, 15 min Wash 0° Fluorescein labelled goat antirabbit IgG, 0°, 15 min Wash 0° View. FITC, Fluorescein isothiocyanate. TABLE II. Inhibition of Immunofluorescence ... 

Fluorescein labelled-anti-H-2.4 IgG at 0°, 15 min Wash 0° View. FITC, Fluorescein isothiocyanate. 

FITC fluorescein isothiocyanate ssDNA single-stranded DNA... 

Abbreviations used FITC = fluorescein isothiocyanate DTAF = dichloro-triazinylaminofluorescein, TRITC = tetramethylrhodamine isothiocyanate, RB-200SC s lissamine rhodamine sulfonylchloride, RBITC = rhodamine B isothiocyanate, XRITC = rhodamine X isothiocyanate CY3.18 = cyanine 3.18, B-PE = B phycoerythrin, R-PE = R phycoerythrin SITS = 4-acetamido-4 -isothiocyanatostilbene-2,2 -disulfonic acid, DAMC = diethylaminocoumarin, AMCA = 7-Amino-4-methylcoumarin-3-acetic acid. Information obtained from refs. (2,9,10)... 

PAHs polycyclic aromatic hydrocarbons. FITC fluorescein isothiocyanate. DNS dansyl halide. OPA o-phthaldialdehyde. NBD 4-clair-7-nitrobenz-2-oxa-1,3-diazole. YOYO-1 benzoxazolium-4-quinolinium dimer. NAD" nicotinamide adenine dinucleotide. 

FITC fluorescein isothiocyanate a fluorochrome that fluoresces greenish yellow. 

Abbreviations CHO, cholesterol DPPC, dipalmitoylphosphatidylcholine FITC, fluorescein isothiocyanate GAS, gas antisolvent POPC,... 

FITC (fluorescein-5-isothiocyanate) Fluor 488 494 518 Yellow-green (12, 13, 18, 34, 38, 39)... 

Abbreviations. FITC, fluorescein isothiocyanate D, dextran BSA, bovine serum albumin IgG, immunoglobulin G. 

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