Fibronectin-binding proteins

Hanski, E., Horwitz, P. A., and Caparon, M. G. (1992). Expression of protein F, the fibronectin-binding protein of Streptococcus pyrogenes JRS4, in heterologous streptococcal and enterococcal strains promotes their adherence to respiratory epithelial cells. Infect. Immun. 60, 5119-5125. 

Natanson, S., Sela, S., Moses, A. E., Musser, J. M., Caparon, M. G., and Hanski, E. (1995). Distribution of fibronectin-binding proteins among group-A streptococci of different M-types. /. Infect. Dis. 171, 871-878. 

Schwarz-Linek, U., Hook, M., and Potts, J. R. (2006). Fibronectin-binding proteins of grampositive cocci. Microbes Infect. 8, 2291-2298. 

Staphylococcus aureus Cowpea D2 peptide of fibronectin-binding protein (EuBP) Brennan et ah, 1999... 

Dziewanowska K, Carson AR, Patti JM, Deobald CF, Bayles KW, Bohach GA Staphylococcal fibronectin binding protein interacts with heat shock protein 60 and integrins role in internalization by epithelial cells. Infect Immun 2000 68 6321-6328. 

Fischer JR, LeBlanc KT, Leong JM. Fibronectin binding protein BBK32 of the Lyme disease spirochete promotes bacterial attachment to glycosaminoglycans. Infect Immun 2006 74 435 41. 

Oh KB, Oh MN, Kim JG, Shin DS, Shin J (2006) Inhibition of sortase-mediated Staphylococcus aureus adhesion to flbronectin via fibronectin-binding protein by sortase inhibitors. Appl Microbiol Biotechnol 70 102-106... 

Innocentin, S., Guimaraes, V., Miyoshi, A., et al. (2009) Laclococcus lactis expressing either Staphylococcus aureus fibronectin-binding protein A or Listeria monocytogenes internalin A can efficiently internalize and deliver DNA in human epithelial cells. Appl Environ Microbiol 75, 4870-4878. 

Pontes, D., Innocentin, S., Del Carmen, S., et al. (2012) Production of Fibronectin Binding Protein A at the surface of Lactococcus lactis increases plasmid transfer in vitro and in vivo. PLoS One 7, e44892. 

A DNA vaccine involving the Ag85A gene which encodes a major secreted fibronectin-binding protein of M marinum and cloned in a eukaryotic expression vector stimulated a protective humoral immune response in hybrid striped bass when administered i.m. (RPS = 80% and 90% depending on dose) (Pasnik and Smith, 2005,2006). 

Arnqvist, A., Olsen, A., Pfeifer, J., Russell, D. G., and Normark, S. (1992). The Crl protein activates cryptic genes for curli formation and fibronectin binding in Escherichia coli HBIOI. Mol. Microbiol. 6, 2443-2452. 

Matsui H, Takahashi T. Mouse testicular leydig cells express klk21, a tissue kallikrein that cleaves fibronectin and igf-binding protein-3. Endocrinology 2001 142 4918-4929. 

In analogy with constrained peptide libraries, several reports have described the use of small proteins, protein domains, or antibodies as scaffolds for the display of random polypeptide sequences to obtain novel binding proteins or antibodies. Koide et al. (81) used the tenth FN3 sequence, a 94-amino-acid fibronectin domain (82, 83) known to be involved in molecular recognition, as a scaffold to build a phagemid 3+3 library L7 (Fig. 10.16) where less than a copy of modified FN3 was present on each phage capsid. The 10 -member library was screened using plates coated with ubiquitin, a small protein for which native FN3 does not have any affinity. The library was made by randomizing five amino acids in positions 26-30 (BC) and five amino acids in... 

The most unusual property of OPN is imparted by the presence of the RGD sequence, which explains the affinity of OPN for integrin and the fact that, hke other integrin-binding proteins (e.g. fibronectin and vitronectin), OPN is often found in association with the extracellular matrix. Consequently, the best-documented biological activities of OPN are its ability to promote adhesion and migration in many types of cells, which appears to depend on the presence of the integrin-binding RGD sequence. 

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