Phages capsid

Cesareni G, Peptide display on filamentous phage capsids, A new powerful tool to study protein-ligand interaction, FEBS Lett., 307(l) 66-70, 1992. 

In analogy with constrained peptide libraries, several reports have described the use of small proteins, protein domains, or antibodies as scaffolds for the display of random polypeptide sequences to obtain novel binding proteins or antibodies. Koide et al. (81) used the tenth FN3 sequence, a 94-amino-acid fibronectin domain (82, 83) known to be involved in molecular recognition, as a scaffold to build a phagemid 3+3 library L7 (Fig. 10.16) where less than a copy of modified FN3 was present on each phage capsid. The 10 -member library was screened using plates coated with ubiquitin, a small protein for which native FN3 does not have any affinity. The library was made by randomizing five amino acids in positions 26-30 (BC) and five amino acids in... 

The organization of the dsDNA within phage capsids has long intrigued... 

Gastroenteritis, Norwalk virus (NV) and, 25 Generic phage capsid assembly pathway, 302, 303 Genome capsid layer organization, in double-stranded RNA viruses, 230-232... 

Cekritelli etal., Fig. 1. (a) Generic phage capsid assembly pathway. This diagram... 

The purified tailspikes can bind to phage capsids in vitro and make the resulting phage particles infectious (42). The thermal unfolding intermediate was unable to bind to the phage capsid (40). The specific binding activity to phage... 

Arsuaga J, Vazquez M, Trigueros S, Sumncas D, Roca J (2002) Knotting probability of DNA molecules confined in restricted volumes DNA knotting in phage capsids. Proc Natl Acad Sci 99 5373-5377... 

The morphogenesis of 29 phage capsid is under the control of genes J, G, A and E (Fig. 3). Cistron G codes for the major capsid proteins HPl and/or HP3 and, probably for the DNA-associated protein, whereas cistrons E and A determine the upper collar protein (NP2), and the tail protein (TPl), respectively. 

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