Fibroblasts micrograph

Fig. 5.8 TEM micrographs of fibroblast cells cultured in the absence (A) or presence (B—D) of silica/alginate nanocomposites (Adapted from [34]).

Figure 7-32 Micrograph of a mouse embryo fibroblast was obtained using indirect immunofluorescence techniques.313 The cells were fixed with formaldehyde, dehydrated, and treated with antibodies (formed in a rabbit) to microtubule protein. The cells were then treated with fluorescent goat antibodies to rabbit /-globulins (see Chapter 31) and the photograph was taken by fluorescent light emission. Courtesy of Klaus Weber.

Figure 8-1 Electron micrograph of a thin section of a fat storage cell or adipocyte. L, the single large fat droplet N, nucleus M, mitochondria En, endothelium of a capillary containing an erythrocyte (E) CT, connective tissue ground substance which contains collagen fibers (Co) and fibroblasts (F). The basement membranes (BM) surrounding the endothelium and the fat cell are also marked. From Porter and Bonneville.6 Courtesy of Mary Bonneville.

Electron micrograph of LDL particles (made electron-dense with covalently bound ferritin) bound to coated regions of a human skin fibroblast (97,000 X ). (From R. G. W. Anderson, M. S. Brown, and J. L. Goldstein, Role of the coated and endocytic vesicle in the uptake of receptor-bound low-density lipoprotein in human fibroblasts. Cell 10 351, 1977. Cell Press.)... 

Specifically, the data reviewed in Chapters 12 and 13 indicate that LCM are rapidly removed from the circulation by the tumor the maximum accumulation of LCM in the tumor area occurs within the first 30 min after administration (ref. 531). These rapid kinetics for LCM uptake are quite consistent with the well-documented kinetics long-known for the LDL receptor-mediated endocytic pathway (ref. 616). For example, Goldstein et al. (ref. 643) reported that LDL-ferritin bound to coated pits at 4°C is rapidly internalized when fibroblasts in tissue culture are warmed to 37°C. In this uptake process, the coated pits invaginate to form coated endocytic vesicles. After 5 to 10 min at 37°C, LDL-ferritin is observed in lysosomes as the result of their fusion with the incoming coated vesicles (ref. 643). The rapid sequence of events visualized in electron micrographs precisely parallels biochemically-derived data on the rapid uptake and degradation of radiolabeled LDL (ref. 644,645). 

Fig. 1. The accuracy of e-beam lithography is illustrated in the scanning electron micrograph (top). The size of the features formed in the silicon oxide is 0.5 pm and the typical animal cell (a fibroblast) has a diameter of 20 pm. This kind of cell adheres actively to surfaces, forming thin filopodia which here have all attached to the micro-hillocks. Semiconductor technology is capable of manufacturing micro-electrodes, sensors, pores and electronic networks with sizes smaller than that of the cell. The lower illustration summarises the main detection and measuring methods currently in use...

Fig. 11. Scanning electron micrographs (a-d) shown sequential stages in the early part of the adhesion process for mouse fibroblasts from initial contact with a surface to the assumption of a more or less final morphology. The cytoskeleton has the ability to change cell shape quickly and an individual cell may pass from the initial spherical form to the final flattened one in a few minutes. The initial adhesion process at the points of contact between cell and surface is also very rapid but there are subsequent changes at the adhesion sites affecting the nature and strength of the bonds which may continue for many hours. These can be studied by TIRF microscopy...

Fig. 17. a A scanning electron micrograph of square pores etched in a 3 micrometer thick silicon membrane. The pores were produced by anisotropic etching and their width on this side of the membrane is 6 pm. Cells (fibroblasts 3T3) attach to the surface and migrate over the pores, b Electrodes are placed on either side of the membrane and a constant current passed through it (mainly through the pores). The presence of cells is easily detected and movements of cell filopodia of less than 100 nm and the passive electric properties of the cell body can be determined by analysis of the signal fluctuations and impedance... 

Figure 26.17 Endocytosis of LOL bound to its receptor. (A) Electron micrograph showing LDL conjugated to ferritin for visualization, dark spots) bound to a coated-pit region on the surface of a cultured human fibroblast cell.

A FIGURE 5-32 Fluorescence micrograph of a PtK2 fibroblast cell stained to reveal keratin intermediate filaments. A network of filaments crisscrosses the cell from the nucleus to the plasma membrane. At the plasma membrane, the filaments are linked by adapter proteins to two types of anchoring junctions desmosomes between adjacent cells and hemidesmosomes between the cell and the matrix. [Courtesy of R. D. Goldman.]... 

Cultured fibroblasts were stained with a fluorescent antitubulin antibody (green) and the DNA-binding dye ethidium (purple). Thus in these fluorescence micrographs, green reveals microtubules purple, chromatin and blue, regions with both structures, (a) During early prophase, the nucleus is surrounded by an array of interphase microtubules and the chromatin is diffuse, (b) By prometaphase, the nuclear... 

A scanning electronic micrograph of a fibroblast and collages fibers. Collagen is a major component of connective tissue. 

Figure 4.26 Intracellular structure, (a) Illustration of the internal structure of a typical human cell, (b) Fluorescence micrograph showing the organized actin filaments in a surface-attached fibroblast.

FIGURE 2.2 Fluorescence micrographs of fibroblast cells cultured on PHBV nanofiber scaffolds after 48h of culture. 

Figure 2. Hyperamplification of the centrosome in PARP-1 -/- mouse embryonic fibroblasts, a-d) normal mouse embryo fibroblast (MEF). e,0 PARP-1 -/- MEF. a) Electron micrograph of the centrosome, consisting of a pair of centrioles situated perpendicular to each other, b-f) The centrosomes were stained in red, the microtubules in green and DNA in blue. b,c) One or two centrosomes were found in the interphase, d) In mitosis the centrosomes translocate to each pole and become the spindle poles, e) Abnormal numbers of the centrosomes were found in PARP-1 -/- MEF. 0 Abnormal spindles were found in mitoses of PARP-1 -/- MEF.

Figure 18.4 TEM micrographs of (A) necrotic human fibroblast cell (HFF-l) cell exposed to free branched PEI (3 j,M in 2 mL media for 3 h) and (B) HFF-l cells exposed to PEI (MW 25 000 kDa) conjugated quantum dots. 3 nM concentration in 2 mL media for 24 h caused limited membranal disruption however, 6 liM concentration in 2 mL media for 3 h caused widespread membranal blebbing.

Fig. 17. Electron micrograph of a Chinese hamster fibroblast in prophase. Portions of two chromosomes enclosed within the nuclear envelope are shown in one the two sister kinetochores I ki/ "k2") are visible note their "back-to-back" arrangement. X60,000. The material was not treated with colcemid Icf. Brinkley and Stubblefield, 1966) glutaraldehyde fixation, post-fixed in 0s04, uranyl and lead staining. (Unpublished micrograph courtesy of B. R. Brinkley.)...

Kg. 14 Optical micrograph showing the adhesion of 3T3 fibroblast cells on a MUA-MUD-derived FN-BSA gradient prepared by electrochemical desorption. The top and bottom x-axes show the spatial/potential... 

Figure 22.1 (a) Light micrograph demonstrating fibroblast and collagen filling of a matrix pore. Coseeded keratinocytes can be seen in the upper right comer, (b) Hematoxylin and eosin-stained section of composite cultured skin development. The final result is a bilayered skin inside each polymer foam pore, (c) The same section as in (b), stained with anti-bovine keratin antibodies, to confirm that the keratinocytes and fibroblasts had adopted layers within the polymer pores. 

F. 8 SEM micrograph showing the L929 fibroblasts adhesion to the surface of SEVA-C (a, b) and SCA (c, d) polymers, a, C One day of growth b, d Seven days of growth. Reprinted with ptaroission Marques et al. (2002), Copyright 2(K)2 Elsevier... 

Figure 6.11 Micrographs of tissue section 3 days after Hyakepair-10 injecUon. The boundary zone between fat tissue and gel is shown. An increased number of fibroblasts and fully developed blood capillaries are detected. Magnification 400x...

Figure 6.12 Micrographs of tissue section 7 days after Hyalrepair-02 injection. The upper part of the image shows the gel with ingrown fibroblast and blood vessels. The lower part shows the fat tissue. Connective capsule surrounding the gel implant is not detected...

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