Fibroblast loading

Binder M, Liebisch G, Langmann T, et al. (2006) Metabolic profiling of glycerophospholipid synthesis in fibroblasts loaded with free cholesterol and modified low density hpoproteins. J Biol Chem 281 21869-21877... 

Column Chromatography. Sepharose beads containing covalently linked gangliosides (0.2 ml packed volume) were placed into a pasteur pipette containing a small amount of glass wool. Columns were washed with HEM containing 50 ug/ml bovine serum albumin (3 ml). Interferon solutions in MEM-albumin (1 ml) were placed on the columns, which were eluted with MEM-albumin at a flow rate of no more than one drop per minute. Fractions of 1 ml were collected and interferon titers determined in each fraction after serial two-fold dilution. Columns onto which mouse fibroblast interferon had been loaded, were eluted with MEM-albumin first, then with 0.07 M N-acetylneuraminyl lactose at pH 2. 

Butt, R.P., and Bishop, J.E. 1997. Mechanical load enhances the stimulatory effect of serum growth factors on cardiac fibroblast procollagen synthesis. J. Mol. Cell. Cardiol. 29 1141-1151. 

ECMs contain a variety of cell types (see Table 1.2) such as fibroblasts in the skin, chondrocytes in cartilage, and osteoblasts in bone. The role of these cells is to synthesize and deposit the ECM surrounding the cell and to change the amount and location of the ECM in response to trauma and changes in external loading. For instance, collagen synthesis in these cells is typically up-regulated by the application tensile forces and is down-... 

Fibroblasts Dense, aligned connective tissue Supports loads... 

Skeletal muscle is composed of muscle cells, thin and thick filaments, and endomysium, a connective tissue containing fibroblasts that holds the fibers together. Mechanical loading regulates normal muscle metabolism in the absence of normal tensile loading muscle atrophy results. Interactions between thick (myosin) and thin (actin) filaments result in lines or bands containing one or more of the muscle fiber components. The H band represents overlap of only the thick filaments whereas the I band represents the area of overlap of thin and thick filaments. The z lines are the points at which the sarcomere repeats itself. 

Tendons and ligaments are made up of units termed fascicles that are bound into functional units by a sheath termed epitendineum (Figure 3.22). Individual fascicles are composed of rows of fibroblasts that alternate with bundles of collagen fibrils parallel to the tendon axis. These structures are normally loaded in tension to maintain joint stability. 

In skin, both epidermal (keratinocytes) and dermal cells (fibroblasts) respond to mechanical loading by undergoing structural and biochemical changes. Wang and Stamenovic (2000) have shown that fibroblasts grown... 

Brown RA, Prajapati R, McGrowther DA, Yannas IV, Eastwood M. Tensional homeostasis in dermal fibroblasts Mechanical responses to mechanical loading in three-dimensional substrates. J Cell Physiol. 1998 175 323-332. 

Eastwood M, Mudera VC, McGrouther DA, Brown RA. Effect of precise mechanical loading on fibroblast populated collagen lattices Morphological changes. Cell Motil Cytoskeleton. 1998 40 13-21. 

Grinnell F. Fibroblast-collagen-matrix contraction Growth-factor signaling and mechanical loading. Trends Cell Biol. 2000 10 362-365. 

Grinnell F, Ho C-H. Transforming growth factor (3 stimulates fibroblast-collagen matrix contraction by different mechanisms in mechanically loaded and unloaded matrices. Exp Cell Res. 2002 273 248-255. 

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