Methanol, extraction using

The step 1 product (0.412 g) was dissolved in 10 ml of chlorobenzene and treated with iron(III) chloride (0.46 g) in 10 ml of chlorobenzene and then heated to 65°C for 48 hours. After cooling to ambient temperature 15 ml of chlorobenzene was added and the solution poured into 200 ml methanol where it was ultrasonicated for 2 minutes. After being stirred at ambient temperature for 1 hour, a precipitate was isolated that was added to a mixture of 200 ml of methanol and 50 ml 12 M ammonium hydroxide solution. The mixture was then ultrasonicated for 30 minutes and a dark red solid precipitated. The solid was purified by Soxhlet extraction using methanol for 3 hours, hexane for 24 hours, heptane for 24 hours, and then chlorobenzene for 24 hours. After filtration 0.12 g of product was isolated as a dark red solid having an M = 16,550 Da, Mw = 65,300 Da, with a polydispersity of 3.95. 

Table V lists the common solvents used for cleaning XAD resins and their respective solubility parameters. Methanol and acetonitrile [which are used in the cleanup procedure of Junk et. al. (5)] have very different total solubility parameters than the compounds listed in Table IV. Thus, they should be less efficient for eluting the resin contaminants from the resin polymers. This situation explains the GC profile results, which show large numbers and high concentrations of contaminants after the successive 24-h Soxhlet extractions using methanol, acetonitrile, and ethyl ether (Figure 2).

SFE and SFE/derivatization for the extraction of phenols from a coal gasification wastewater have been performed by first collecting the wastewater organics on "Empore" C-18 sorbent discs (8). The sorbent discs were first prepared by washing with methanol and water as per the manufacturer s instructions. A 100-mL sample of wastewater was acidified to a pH <2, 4 mL of methanol was added, and the waters were filtered through the sorbent discs. The discs were then placed in a 2.5 mL cell and extracted at 80°C and 500 atm using pure C02, C02 with 1.0 mL methanol modifier placed in the cell, or C02 with 1 mL of 0.1 M TMPA in methanol placed in the cell. (Extractions using methanol modifier and TMPA derivatization were each performed statically for 5 minutes followed by dynamic extraction for 10 minutes.) No attempt was made to dry the discs before SFE. 

Fractionation with organic solvents is mainly done to remove proteins, large peptides, and non-proteinaceous material such as fat. In a method developed by Harwalkar and Elliott (1971) and adopted by Lemieux et al. (1990), Puchades et al. (1990), and Visser et al. (1983), freeze-dried samples of cheese were extracted using methanol (to precipitate large peptides and proteins), chloroform (to remove fat), and water. The final extract... 

Atrazine, simazine, terbuthylazine, molinate Solid-phase microwave-assisted extraction using methanol Gas chromatography-mass spectrometry. Limit of detection 1-10 ng/g [382]... 

Appraising the toxic potential of biologically available contaminants in sediment should include three compartments the whole sediment (with standardized direct contact assays when these are available), the porewater, and the elutriate (aqueous extract). Additional hazard information can also be obtained from toxicity testing conducted on organic extracts using methanol or acetone. 

The polymer is then purified by Soxhlet extraction using methanol for 6-8 h. Finally, the polymer is dried in a vacuum oven at 40°C for 24 h. ... 

Food samples were used for carbaryl screening and determination. Carbaryl was spiked at three levels in samples and extracted using methanol, which was earlier found to be an efficient extractant for this compound in food and environmental matrices. The samples were screening by lateral-flow gold-based immunoassay and confirming by HPLC (shown in Table 3). 

We have derived an extraction method that retains the convenience of the ball-mill tumbler for large numbers of extractions, and have investigated various solvent systems for improved recoveries. Preliminary results indicate that a tumbler extraction using methanol and dichloro-methane recovered hydrocarbons from an intertidal sediment as efficiently as the soxhlet technique (12). This report describes improvements in our tumbler procedure and compares it with three boiling-solvent procedures (10,13,15). 

Some components of organic phosphorus may also be extracted using organic solvents. Phospholipids, for example, which are used as biomarkers for microbial communities, can be extracted using methanol-chloroform-water (White et al., 1979), by pressurized hot solvent extraction (Macnaughton et al., 1997), cold methanol-water, or a surfactant such as Triton X-100 (Amini, 2001). 

Hydrochloric add doped pol aniiine (HCl-PANi) was synthesized by a standard method (29% dedoped with ammonium hydroxide and So dilet extracted using methanol for 72 hours. The extracted sanq>le was dried under vacuum ovemig and used for characterization studies. 

NiP-CPE DPV Potato flakes Liquid-liquid extraction using methanol/water (50% v/v) followed by pre-concentration in a vacuum rotary evaporator. BHA Ruiz et al. (1994)... 

CCE and Pt disk SWV Mayonnaise Liquid-liquid extraction using methanol followed by centrifugation. BHA, BHT, and TBHQ Raymundo et al. (2007)... 

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