Expression vectors discovery

We may also find that Sabatini s reverse transfection method of creating "live cell" microarrays offers even greater advantage for drug discovery and development (Ziauddin and Sabatini, 2001). Their method, relying on arrayed cDNA expression vectors to transform adherent cells, provides localized real-time gene expression analysis of the putative gene product. 

While the initial goal of the discovery process is to express and purify the recombinant protein, without regard to cost and as quickly as possible, ultimately the production of a recombinant protein must be optimized to ensure that it can be produced at a reasonable cost. In most cases expression systems used to produce small quantities of recombinant protein for preliminary evaluation are not suitable to produce large quantities. Therefore, the gene inserted in the initial expression vectors must be systematically modified and recloned into vectors that can produce (1) a high yield of expressed protein, (2) stable host cell transfections, and (3) protein excretion. 

The technology platform is categorized within three areas Viral vector technologies (for gene expression), Biomanufacturing resources (for protein production) and Molecular improvement and medical discovery technologies (for discovery of biomolecules, genes and proteins, and the improvements of their functionality). 

Arts, G.-J et at. Adenoviral vectors expressing siRNAs for discovery and validation of gene function. Genome Res. 2003, 13, 2325-2332. 

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