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Evaporative light scattering detection , analysis

Peterson, J. A., Lorenz, L. J., Risley, D. S., and Sandmann, B. J., Amino acid analysis of peptides using HPLC with evaporative light scattering detection, /. Liq. Chromatogr. Related Technol., 22, 1009, 1999. [Pg.306]

Detection in 2DLC is the same as encountered in one-dimensional HPLC. A variety of detectors are presented in Table 5.2. The choice of detector is dependent on the molecule being detected, the problem being solved, and the separation mode used for the second dimension. If MS detection is utilized, then volatile buffers are typically used in the second-dimension separation. Ultraviolet detection is used for peptides, proteins, and any molecules that contain an appropriate chromophore. Evaporative light scattering detection has become popular for the analysis of polymers and surfactants that do not contain UV chromophores. Refractive index (RI) detection is generally used with size exclusion chromatography for the analysis of polymers. [Pg.109]

Wei Y, Ding MY. Analysis of carbohydrates in drinks by high-performance liquid chromatography with a dynamically modified amino column and evaporative light scattering detection. Journal of Chromatography A 904, 113-117, 2000. [Pg.228]

Caboni, M.F. and Rodriguez-Estrada, M.T. 1997. High-performance liquid chromatography coupled to evaporative light scattering detection in lipid analysis Some application. Seminars in Food Analysis 2 159-169. [Pg.465]

SL Abidi, TL Mounts, T Finn. A preferred solvent system for high performance liquid chromatographic analysis of soybean phospholipids with evaporative light scattering detection. J Am Oil Chem Soc 73 535-536, 1996. [Pg.284]

In LC/MS analysis of combinatorial libraries, the MS determines the product identity and its purity is determined by other on-line detection techniques such as UV, evaporative light scattering detection (ELSD), and chemiluminescent nitrogen detection (CLND).17-20 UV detection is used here to assess product purity based on the assumption of similar absorption coefficients at 214 nm for the desired product and the side-products. [Pg.12]

Petritis, K. et al. Validation of ion-interaction chromatography analysis of underivatized amino acids in commercial preparation using evaporative light scattering detection. [Pg.105]

Analysis of fatty acid methyl esters by using supercritical fluid chromatography with mass evaporative light-scattering detection S. Cooks and R. Smith, Anal. Proc. 28,11 (1991)... [Pg.184]

HPLC analysis of phospholipids by evaporative light-scattering detection T. L. Mounts, S. L. Abidi, and K. A. Rennick, J. AOCS 69 438 (1992)... [Pg.184]

In contrast, newer techniques such as FID and evaporative light-scattering detection (ELSD, Universal Detector ) are more sensitive and have been used for the analysis of many lipids, including the polar and neutral lipid classes [6]. For preparative scale separations, if the UV absorbance is more than what the detector can handle, an evaporative light-scattering detector is used [B2]. [Pg.924]

Warner, K. and Mounts, T.L. 1990. Analysis of Tocopherols and Phytosterols in Vegetable Oils by HPLC with Evaporative Light-Scattering Detection. J. Am. Oil Chem. Soc. 67 827-831. [Pg.37]

Cremin, P.A. Zeng, L. High-Throughput Analysis of Natural Product Compound Libraries by Parallel LC-MS Evaporative Light Scattering Detection, Anal. Chem. 74, 5492-5500 (2002). [Pg.176]

Our present approach is to perform a large number of analyses to resolve the single desired product or other contaminants, use the target molecular weight to confirm the presence of the product, and generate the relative purity by either ultraviolet detection, evaporative light scattering detection, or some other detection protocol [3]. The samples for analysis are typically presented in 96-well microtiter plate (MTP) format and the result-... [Pg.792]

NPLC methods were reported for the analysis of natural phospholipids in nervous tissues, meat, etc. Mono-, di-, and triacylglycerol classes in natural oils can be distinguished on silica gel columns using normal-phase gradient elution. In these applications, either low-wavelength UV detection at 205 nm, or evaporative light-scattering detection is used for quantitative analysis. [Pg.2570]

Detection systems used for LC analysis of pesticides are UV spectrometric detection (especially diode array detection - DAD - allowing peak confirmation by means of spectra comparison), fluorescence detection (FLD), electrochemical detection (ED), evaporative light scattering detection (ELSD), and MSD. Their characteristic sensitivities can be considered to vary in the following order ELSDdetection systems mentioned above, only ELS and MS are universal detectors. [Pg.3605]

Source From Amino acid analysis of peptides using HPLC with evaporative light scattering detection, in J. Liq. Chromatogr. Relat. Technol. ... [Pg.70]

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