Eukaryotic cell line

Secreted proteins from eukaryotic cell lines present problems in purification due to both the volume of... 

Glycosylation is a possible post-translational modification, characteristic of recombinant proteins expressed from eukaryotic cell lines (see Chapter 6). 

Efflux transporters are often accounted for by the transport of endogenous compounds and pharmaceutical drugs out of the body into bile, urine or gut in order to protect the cell. To study drug efflux, membrane preparations are necessary. Eukaryotic cell lines, tissues as well as insect cells can be used for this application. 

Preparation of Plasma Membrane Vesicles from Eukaryotic Cell Lines... 

Using insect cells instead of eukaryotic cell lines has following advantages a reduced transporter background and higher expression levels. However, one should also consider that the lipid membrane composition and glycosylation mechanism differ in insect cells from eukaryotic cells. 

Recombinant DNA methods have been extensively used to isolate and analyze the sequence of numerous receptors from various mammalian complimentary DNA (cDNA) libraries using the polymerase chain reaction (PCR) technique (98) to clone receptors that can then be expressed in various pro- and eukaryotic cell lines and Xenopus oocyte. Conserved regions in receptors that are involved in ligand binding, coupling to transductional systems and ion channel formation, have thus been identi-... 

Nowadays, the systems of choice for the production of monoclonal antibodies or antibody fragments are either eukaryotic cell lines or, in some cases, prokaryotes. The following sections will highlight certain manufacturing aspects which are linked to these expression systems. 

Rapsch and co-workers have discussed the antimicrobial activities of different peptides [48b]. They developed a rapid and efficient screening procedure to identify and investigate which biologically active peptides, in an immobilised state, could produce an effective antimicrobial surface coating. In addition to this, an antimicrobial surface coating of peptides exhibited no cytotoxic activity against a eukaryotic cell line (Figure 2.6). 

Both xenofuranones were tested against different bacteria, yeast, and eukaryotic cell lines. However, only 54 showed a weak cytotoxic acitivity (IC50 18pg/mL) against eukaryotic cells (L929), whereas no further biological activity was observed. 

Table 1. Eukaryotic cell lines used in this study.

A secondary mechanism of action is a detergent-like effect (103). However, it is unclear whether this merely r esents the cocqierative accumulation of multistate channels or gross multimerisation of cationic peptides in the membrane, and whether this mechanism is relevant to bacterial cell killing, since it has only been demonstrated in nondefinitive experiments in eukaryotic cell lines and model liposomes (103). In contrast, the lysis of bacteria—often at concentrations exceeding the MIC—prob ly arises from the triggering of autolytic enzymes (104). With diese caveats, it is worth considering how cationic peptides interact with membranes. 

Cj encapsulated Damages eukaryotic cell lines, antibacterial... 

The reported potencies for geldanamycin against eukaryotic cell lines range between 20 nM and 5 and the observed variability has been... 

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