Ethylene glycol bis- 2-aminoethyl

Microtubules can be reconstituted in vitro at 37 °C from a solution that contains a physiological mixture of brain tubulin, MAPs, small amounts of guanosine 5 -triphosphate (GTP), magnesium ions, and the calcium-chelating agent EGTA [ethylene glycol-bis(2-aminoethyl ether) N, N -tetraacetic acid]. Tubulin assembly is inhibited by low temperature and by the presence of calcium ions. 

In this laboratory, we also include the metal ion chelators EDTA (ethylene diamine tetraacetic acid binds, e.g., Mg2 1 -ions) and EGTA (ethylene glycol-bis(2-aminoethyl)-Al,iV,iV/,iV/,-tetraacetic acid binds, e.g., Ca2+-ions) in our lysis buffers. These agents help prevent phosphatase action (by the metal ion-dependent phosphatase PP2C, which is not inhibited by microcystin-LR), metal (Ca2+) dependent proteinases, and protein kinases, which require divalent cations such as Mg2 1 (and, in some cases, also Ca2+). We also use a mix of proteinase inhibitors that inhibit a broad range of proteolytic enzymes, including serine and cysteine proteinases. 

Fig. 1. Approximate pH values and ranges for a selection of biochemical and geochemical environments, with their relation to successive pK values for the aminocarboxylate ligand ethylene glycol bis-(2-aminoethyl ether)-tetraacetate, egta.

ETHYLENE GLYCOL BIS(2-AMINOETHYL ETHER)-N,N,N, N -TETRAACETIC acid see EITOOO ETHYLENE GLYCOL BIS(lJ-AMINOETHYL ETHER)-N,N -TETRAACETIC ACID see EITOOO ETHYLENE GLYCOL, BISCHLOROFORMATE see EIQOOO... 

Chelators such as EDTA, nitrilotriacetic acid (NTA), 1,2-aminocyclohexane 7V,7V,7V ,N7-tetraacetic (DCyTA), and ethylene glycol-bis(2-aminoethyl)-(V,(V, 7V ,7V -tetraacetic acid (EGTA) have been studied extensively and are well summarized (Peters, 1999). Chelator concentration and reaction pH influence metal complexation and the success of removal from soils. Sun et al. (2001) observed that batch extraction methods result in 1 1 molar extraction ratios of EDTA/metal (Pb, Cd, Zn, Cu) and reveal which metal is more or less soluble in EDTA solutions. Column leaching studies, however, relate the elution patterns and recalcitrance of the metals to desorption and dissolution by EDTA. There is concern over the detrimental effects on soil quality from using chelators because of their biotoxicity, persistence in soil environment, and their removal of beneficial micro-and macronutrients, which leave the washed soil infertile for revegetation when it is backfilled. 

Remarks EGTA= ethylene glycol bis(2-aminoethyl ether)-N,N,N, N -tetraacetic acid assignments for AH2 resonances are tentative assignments of both pairs of AH2 /2" resonances probably should be reversed (compare [86P1] for cs data on the same sequence). 

Ethylenebis oxyethylenenitrilo)tetraacetic acid, SCI. Ethylene glycol bis[2-[bis carboxymethyl)amino]ethy[ ether. Ethylene glycol bis 2-aminoethyl ether)- N,N,W, -tetraacetic acid. EGTA. Egtazic acid, INN, US AN [67-42-5]... 

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