Ethylene glycol-bis

Amino Alcohols. Reaction of chloroformate is much more rapid at the amino group than at the hydroxyl group (4—8). Thus the hydroxy carbamates, which can be cyclized with base to yield 2-oxazoHdones, can be selectively prepared (29). Nonionic detergents may be prepared from poly[(ethylene glycol) bis(chloroformates)] and long-chain tertiary amino alcohols (30). 

Microtubules can be reconstituted in vitro at 37 °C from a solution that contains a physiological mixture of brain tubulin, MAPs, small amounts of guanosine 5 -triphosphate (GTP), magnesium ions, and the calcium-chelating agent EGTA [ethylene glycol-bis(2-aminoethyl ether) N, N -tetraacetic acid]. Tubulin assembly is inhibited by low temperature and by the presence of calcium ions. 

An alkylamine linker is incorporated into either the 5 or 3 end of the capture probe and the probe is activated with ethylene glycol bis-succinimidylsuccinate. The activated capture probe is bound to polystyrene microtiter wells precoated with poly(Lys-HBr, Phe). Nearly all of the solid-phase probe coupled to the polystyrene by this procedure is available for hybridization (Running and Urdea, 1990). 

In this laboratory, we also include the metal ion chelators EDTA (ethylene diamine tetraacetic acid binds, e.g., Mg2 1 -ions) and EGTA (ethylene glycol-bis(2-aminoethyl)-Al,iV,iV/,iV/,-tetraacetic acid binds, e.g., Ca2+-ions) in our lysis buffers. These agents help prevent phosphatase action (by the metal ion-dependent phosphatase PP2C, which is not inhibited by microcystin-LR), metal (Ca2+) dependent proteinases, and protein kinases, which require divalent cations such as Mg2 1 (and, in some cases, also Ca2+). We also use a mix of proteinase inhibitors that inhibit a broad range of proteolytic enzymes, including serine and cysteine proteinases. 

Fig. 1. Approximate pH values and ranges for a selection of biochemical and geochemical environments, with their relation to successive pK values for the aminocarboxylate ligand ethylene glycol bis-(2-aminoethyl ether)-tetraacetate, egta.

Armstrong CM, Bezanilla FM, Horowicz P 1972 Twitches in the presence of ethylene glycol bis(-aminoethyl ether)-N,N -tetraacetic acid. Biochim Biophys Acta 267 605-608 Bezprozvanny I, Watras J, Ehrlich BE 1991 Bell-shaped calcium-response curves of Ins(l,4,5)Py and calcium-gated channels from endoplasmic reticulum of cerebellum. Nature... 

Ethylene glycol bis(methallyl carbonate), properties of, 2 253t... 

CHES 3-(Cyclohexylamino)ethanesulfonic acid EGTA Ethylene glycol bis(/3-aminoethyl ether)-A,A,A, A -tetraacetic acid or its conjugate base... 

R Poly(ethylene glycol) Bis-(6- methylsulfinyl)hexanoate). 

EGTA ethylene glycol bis(-aminoethyl ether) tetraacetate... 

Ethylene glycol bis(B-aminoethylether)-V,V -tetraacetic acid (EGTA) [67-42-5] M 380.4, m >245 (dec). Dissolved in aq NaOH, pptd by addn of aq HCl, washed with water and dried at 100° in vacuo. ueous 5% Na2CO3, dried with MgSO4 and stored with chromatographic alumina to prevent peroxide formation. 

Ethylene bis(oxyethylenenitrilo)]tetraacetic acid see ethylene glycol bis(B-aminoethylether)-tetraacetic acid. 

The same concept was used in polycondensation systems for the preparation of branched and cross-linked polymers [121]. Copolyurethanes 73 were prepared from tetra(ethylene glycol), bis(5-hydroxymethyl-l,3-phenylene)-32-crown-10 (74)... 

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