Ethylene diamine tetraacetate, determination

Electrostatic ion chromatography, applications of 99-100 determination of anions 99-101 general discussion 4, 5 Ethylene diamine tetraacetate, determination of 112,113,291... 

Vickery and Vickery [9] have investigated the interference by aluminium and iron in the ion-selective electrode method for the determination of fluoride in plant extracts. They demonstrated that plant ashes may contain sufficient of these two elements to seriously interfere in the determination of fluoride when using the fluoride-selective electrode. In the presence of these metals, the known additions method gives erroneous results, as did that involving the attempted formation of complexes with ethylene diamine tetraacetic acid (disodium salt) or 1,2-cyclohexylenedinitrilotetraacetic acid. 

Other organic compounds that have been determined in sewage effluents include the following (see Table 15.13) hydrocarbons, alcohols, carboxylic acids, esters, chlorobenzenes, nitrosamines, ethylene diamine tetraacetic acid, nitriloacetic acid, organophosphorus compounds, linear alkyl benzene sulphonates, methyl mercaptan, polychlorobiphenyls and chlorinated insecticides. 

Further applications of the determination of gas chromatography to the determination of anions in non saline waters are reviewed in Table 15.23. These include aminoacetates, arsenate, bromide, chloride, fluoride, iodide, cyanide, ethylene diamine tetraacetate, nitrate, phosphate, thiocyanate and sulphide. 

The measurement of endogenous ACTH concentrations is valuable in the diagnosis of ECD (Sojka Levy 1995). Plasma samples for ACTH determination require special handling blood must be collected in chilled disodium ethylene-diamine-tetraacetic acid (EDTA) tubes, maintained at 4°C, centrifuged promptly and the plasma stored at -70°C until analysis. The ACTH reference range may vary between laboratories. There can be an overlap in the values of stressed normal horses and early cases of ECD. 

The cytotoxicity assay is usually performed by determining the viability of suitable cell lines in the presence of polymers. For this test, 3-5 mm discs of polymer film are cut and sterilised under standard conditions (at 121 C and 6.8 kg (15 lb) pressure for 15 min). The cell growth in the presence of the polymer films is measured under a controlled atmosphere (CO2 incubator, 37"C) using an appropriate culture medium, supplemented by 10% fetal bovine serum and penicillin-streptomycin antibiotic solution. Confluent monolayers are propagated by trypsinisation (0.25% trypsin and 0.02% EDTA, ethylene diamine tetraacetic acid) and re-plated at 2 x 10 cells/mL in a sterile polystyrene cell culture plate, then incubated for 24,48 and 72 h. The morphology of the cells is analysed by light naicroscopy (Leica) after... 

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