Slide containers

Shell Chemical Company), exhibits a maximum at 300 nm, corresponding to that of the model chromophore anisole. The fluorescence intensity decreases monotonically with increasing concentration of 2,4-dihydroxybenzophenone (DHB) and, furthermore, decreases with time on continued excitation (274 nm) in the spectrophotometer. The fluorescence loss with time may be resolved into two exponential decays. Initially, a relatively rapid fluorescence loss is observed within 20 sec, followed by a slower loss. Loss constants for the initial (k ) and secondary (kj) exponential decays for 1.5 ym films (on glass slides) containing varying concentrations of DHB are provided in Table I (entries 1-3). The initial loss constants are seen to decrease more markedly with increasing DHB concentration than the secondary constants. 

The utility of a process control was demonstrated by constructing a series of slides containing mouse and rabbit serum. Five rows of a series of dilutions of each species were applied to slides, and these were then stained in mixed cocktails of goat anti-rabbit and goat anti-mouse secondary antibody, followed by routine peroxidase-DAB visualization steps. The mixed cocktails were constructed as variable mixtures to demonstrate the ability to detect lack of sensitivity of either of the cocktail components. The results of this study were published in abstract form.15 Clearly, this approach can provide a way to monitor changes in secondary antibody cocktails, either due to manufacturing variables or differential aging of components within the cocktail (Fig. 10.5). 

Slide container. A plastic Coplin jar was used at the first experiment of AR-IHC in early 1990s. It is still used when testing a few slides. Recently, larger plastic containers are used to contain more slides and AR solutions. 

Glass slide containing Biotin-modified slide via... 

Plastic Coplin jars or other suitable microwaveable slide containers. 

Some or all prehybridization steps are optional, depending on the target and specimen. Prehybridization entails soaking the slides containing the specimens in a prehybridizafion buffer in order to have the specimen rehydrate and equilibrate with the hybridization buffer, to be used later. The prehybridization phase may also entail treatment with proteases or nucleases in order to improve probe access and to reduce background. 

Measnrement Uncertainty. The slide contains the (newer) definition in VIM. In GUM it is defined as ... 

The following slides contain the details of the information that usually have to be included in the test or calibration certificate. These include the date of the measurement, the result, the staff responsible for carrying out the work and where relevant a statement that the results apply to the items tested or calibrated. 

Figure 6.2 Overview slide contains a road map to coming attractions. Road maps are needed at...

Target Retrieval Solution (Dako S1700) is diluted 1 10 with distilled water and adjusted to pH 6.0. ThinPrep smears are rehydrated in 95% ethanol and distilled water for 5 min each. The smears are placed in a plastic slide container with sufficient retrieval solution. The container is immersed in a sufficient quantity of warm water inside the microwave pressure cooker (Dako, DO300X) and heated in a microwave oven for 20 min at full power until steam is generated. The smears, along with retrieval solution, are allowed to cool for 10 min. The smears are removed and rinsed with warm water, followed... 

Each microglass slide contains eight identical subarrays. There is chip space for 600 microspots per subarray, with spot sizes of approx 200 p and at 300-p inter-... 

After venting pressure, open the lid and remove the slide container from the autoclave. 

Slides containing sections are washed in a saturated solution of sodium hydroxide in ethanol for 5 min to remove the embedding plastic from the sections. After careful washing in ethanol and drying, the sections are ready for measurement. 

Histopathological analysis was performed according to a published procedure by Lipkin. The entire cecum, colon, and rectum of two animals, one each from the control group and the MCM-treated group, were removed and fixed with 10% buffered formalin (12 h), 80% ethanol (12 h), and 95% ethanol (12 h). Representative sections were taken, paraffin embedded, and 4-pm sections cut, mounted into glass slides, and stained with hematoxylin and eosin. Five slides were prepared from each tissue, each slide containing five serial sections. The number of epithelial cells per intestinal crypt over 50 intestinal crypts was counted. The number of crypts containing dysplastic epithelial cells per 50 intestinal crypts was counted. 

After the slides have been removed from their packing they can be placed in the analyzer. Two storage containers are available (Fig. 23) each accommodating 30 slide cartridges. Cooling provides for prolonged storage life of the slides (approx. 1-2 weeks in the analyzer). Since the slide containers are fitted... 

Take images which can be used to perform the appropriate shade correction procedure. Fill the microscope slide, containing the 20 xM observation chamber, with either the 3 pM fluorescein solution or the 200 pM 4-methylumbeliferone solution. Find the correct focal plane by focusing on any particles in the solutions and then find a field without particles. Take, for example, a 10 second image of each fluorochrome using the appropriate fdter set (see Note 10). 

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