Epithelial tissue mammary cells

Johnson, V. G., and Mather, I. H. 1985. Monoclonal antibodies prepared against PAS-I, butyrophilin and GP-55 from guinea-pig milk-fat-globule membrane bind specifically to the apical pole of secretory-epithelial cells in lactating mammary tissue. Exp. Cell Res. 156, 144-158. 

Celis JE, Moreira JM, Cabezon T, Gromov P, Friis E, Rank F, et al. Identification of extracellular and intracellular signaling components of the mammary adipose tissue and its interstitial fluid in high risk breast cancer patients Toward dissecting the molecular circuitry of epithelial-adipocyte stromal cell interactions. Mol Cell Proteomics 2005 4(4) 492-522. 

Choi Y.C., Henrard D.H., Lee I., Ross S.R. (1987). The mouse mammary tumor virus long terminal repeat directs expression in epithelial and lymphoid cells of different tissues in transgenic mice. J. Virol. 61 3013-3019. 

The method described above is a manual protocol for syringe loading of FDxLys into the cytoplasm of endothelial (Fig. lA) and fibroblast (Fig. IB) cells in vitro (Clarke and McNeil, 1992). Substitution of other macromolecules (proteins, dyes, or nucleic acids) for FDxLys will result in their loading into the cell cytoplasm by this procedure. Moreover, this technique is not limited to one or two cell types. A variety of other tissue cultured cells have been loaded in this manner, including normal rat kidney epithelial cells, QT6 cells, mouse mammary tumor cells, human angiosarcoma cells, COS-7 cells, PC 12 cells and chick neuronal cells. 

Fig. 1. Indirect in situ RT-PCR for ras mRNA in normal and neoplastic human breast tissues. (A) and (C) Negative controls for ras message. (B) Detection of ras mRNA signal in normal epithelial cells of mammary duct and lobules. (D) Detection of ras mRNA signal in neoplastic ductal epithelial cells in breast tissue with ductal carcinoma in situ (DCIS).

Mather, I.H., Jack, L.J.W., Madara, P.J., Johnson, V.G. 2001. The distribution of MUC1, an apical membrane glycoprotein, in mammary epithelial cells at the resolution of the electron microscope implications for the mechanism of milk secretion. Cell Tissue Res. 304, 91-101. 

Nickerson, S.C., Keenan, T.W. 1979. Distribution and orientation of microtubules in milk secreting epithelial cells of rat mammary gland. Cell Tissue Res. 202, 303-312. 

The specific induction of PA production in secretory epithelial cells in response to signals for mammary gland involution is envisioned by these authors to initiate involution as follows. Secretion of PA leads to increased levels of extracellular PA, which locally activates plasminogen available in the circulation, tissue fluids and milk. 

Feuerhake, F., Sigg, W., Hofter, E. A., Dimpfl, T., Welsch, U. (2000) Immu-nohistochemical analysis of Bcl-2 and Bax expression in relation to cell turnover and epithelial differentiation markers in the non-lactating human mammary gland epithelium. Cell Tissue Res. 299,47-58. 

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