Electrospray ionization tandem mass spectrometry ESI

Schneiter, R. Brugger, B. Sandhoff, R. Zellnig, G. Leber, A. Lampl, M. Athenstaedt, K. Hrastnik, C. Eder, S. Daum, G. Paltauf, F. Wieland, F. T. Kohlwein, S. D. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J. Cell Biol. 1999,146,741-754. 

Kamel AM, Zandi KS, Massefski WW. 2003. Identification of the degradation product of ezlopitant, a non-peptidic substance P antagonist receptor, by hydrogen deuterium exchange, electrospray ionization tandem mass spectrometry (ESI/MS/MM) and nuclear magnetic resonance (NMR) spectroscopy. J Pharm Biomed Anal 31 1211. 

A newer approach for lipid analysis is electrospray ionization tandem mass spectrometry (ESI-MS/MS) (Welti et al., 2002). This method requires limited sample preparation and sample size to identify and quantify lipids. Fauconnier et al. (2003) used ESI-MS/MS to analyze phospholipid and galactolipid levels during aging of potato tubers. 

Immler, D. Gremm, D. Kirsch, D. Spengler, B. Presek, R Meyer, H. E. 1998. Identification of phosphorylated proteins from thrombin-activated human platelets isolated by two-dimensional gel electrophoresis by electrospray ionization-tandem mass spectrometry (ESI-MS/MS) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). Electrophoresis, 19,1015-1023. 

Liebisch G, Binder M, Schifferer R, Langmann T, Schulz B, Schmitz G. High throughput quantification of cholesterol and cholesteryl ester by electrospray ionization tandem mass spectrometry (ESI-MS/MS). Biochim. Biophys. Acta 2006 1761 121-128. 

With electrospray ionization tandem mass spectrometry (ESI-MS/MS), the liquid sample is introduced into the ion source through a capillary tube and exposed to a strong electric field and a counter flow of nitrogen gas, which in combination produce the electrospray (see Chapter 7). The solvent evaporates and eventually desorption of charged ions occurs into the mass analyzer. ESI occurs under atmospheric... 

Neuromuscular blockers can be analyzed using HPLC with fluorescence, ultraviolet (UV), or electrochemical detection (ECD). As an alternative to HPLC, GC with nitrogen—phosphorus detection (NPD) and LC coupled with electrospray ionization tandem mass spectrometry (ESI-MS-MS) have also been investigated as viable techniques to measure neuromuscular blockers (Table 10.2). 

A study of the catalytic a-acetoxylation reaction of acetophenone by electrospray ionization tandem mass spectrometry (ESI-MS/MS) has confirmed the mechanism shown in Scheme 4.2. In particular, the trivalent iodine species was detected when iodobenzene and mCPBA in acetic acid were mixed, which indicated the facile oxidation of a catalytic amount of Phi to the iodine(III) species by mCPBA. Most importantly, the protonated alkyliodonium intermediate 3 (R = Ph, = H) was observed at m/z 383 from the reaction solution and this ion gave the protonated a-acetoxylation product 1 at m/z 179 in MS/MS by an intramolecular reductive elimination of Phi [17]. 

Solution-phase approaches have been successfully adopted by a number of other groups in order to both discover and optimize olefin polymerization catalysts. Mason and Coates used GPC on polymer mixtures from pooled catalyst libraries in order to identify active heteroligated phenoxyimine titanium catalysts. Hinderling and Chen reported electrospray ionization tandem mass spectrometry (ESI-MS/MS) as a means of identilying palladium catalyst complexes in pooled libraries. Adams a al. analyzed a library of 50 imidotitanium complexes for ethylene polymerization, and identified seven active complexes. Finally, in a study by Jones et a library of 205... 

A sensitive method for quantitation of glimepiride in human plasma was established using electrospray ionization tandem mass spectrometry (ESI/MS/MS). Detection of glimepiride is accurate and precise with a quantitation limit of 0.1 ng/ml. This method was successfully applied to a pharmacokinetic study of glimepiride. No interferences of the analytes were observed because of high selectivity of the MS/MS technique. The method of Song et al. is also sufficiently sensitive, with a quantification limit lower than the minimum concentration recommended for plasma samples obtained after the administration of 2 mg glimepiride. This smdy indicates a reliable stability of... 

Hess, T.F., Renn, T.S., Watts, R.J., Paszczynski, A.J. (2(X)3) Studies on Nitroaromatic Compound Degradation in Modified Fenton Reactions by Electrospray Ionization Tandem Mass Spectrometry (ESI-MS-MS). Analyst 128 156-160. 

Microcolumn reverse-phase HPLC electrospray ionization tandem mass spectrometry (ESI-MS/MS) is a rapid and sensitive technique for the analysis of complex mixtures of peptides. This technique is used to determine the amino acid sequence of unknown peptides, to verify the structure of proteins, and to determine posttrans-lational modifications (see also the article by Beth L. Gillece-Castro). In particular, the strength of this approach is the analysis of peptides in complicated mixtures, such as amino acid sequence analysis of peptides isolated from class I and II major histocompatibility T-cell receptor complexes (Hunt et al., 1992a). 

Liebisch, G., Drobnrk, W., Reil, M., Trumbach, B., Arnecke, R., Olgemoller, B., Roscher, A. and Schmitz, G. (1999) Quantitative measurement of different ceramide species from crude cellular extracts by electrospray ionization tandem mass spectrometry (ESI-MS/MS). J. Lipid Res. 40, 1539-1546. 

MS AA and folic acid are determined. Quantitative detection is achieved by electrospray ionization tandem mass spectrometry (ESI-MS/MS) in negative ion mode using the method of standard addition 5.9 ng g-> No data 0.2-3.1 Pharmaceuticals 2013 253... 

Due to the low volatility and the thermal instability of BA, and in order to address the persistent need for a rapid and sensitive means of BA screening in biological fluids, electrospray ionization-tandem mass spectrometry (ESI-MS/MS) techniques have been proposed for the analysis of these compounds. These methods allow direct analysis of the intact polar forms, either unconjugate or conjugate. 

---