Electrospray ionization mass products

Herbach, K.M., Stintzing, E.C., and Carle, R., Identification of heat-induced degradation products from purified betanin, phyUocactin and hylocerenin by high-performance liquid chromatography/electrospray ionization mass spectrometry, Rapid Commun. Mass Spectrom., 19, 2603, 2005 20, 1822, 2006. 

Naito, Y. Ishikawa, K. Koga, Y. Tsuneyoshi, T. Terunuma, H. Arakawa, R. Molecular mass measurement of polymerase chain reaction products amplified from human blood DNA by electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 1995,9,1484-1486. 

Yiang, Y. Hofstadler, S. A. A highly efficient and automated method of purifying and desalting PCR products by electrospray ionization mass spectrometry. Anal. Biochem. 2003,316, 50-57. 

Zhang and Nunes [92] studied the structure and the generation mechanism of a novel degradation product formed by oxidatively induced coupling of miconazole nitrate with butylated hydroxy toluene in a topical ointment studied by high performance liquid chromatography-electrospray ionization mass spectrometry and organic synthesis. 

Affinity capture-release electrospray ionization mass spectrometry (ACESIMS) is another recently introduced technique for quantification of proteins, and to date has most often been applied to clinical enzymology.60 The product conjugates of the enzymatic reaction between the synthetic substrate and targeted enzyme are captured by immobilized affinity reagents, purified, released into solution, and analyzed by ESI-MS. 

Fig. 2. Individual positive ion electrospray ionization mass spectra taken at time (a) 0 and (b) 10 min into the reaction. The d isappearance of t he peak at m/z 649 and the growth of the products at m/z 191 and 397 are clearly evident.

Y. Yang, K. Janota, K. Tabei, N. Huang, M. M. Siegel, Y. I. Lin, B. A. Rasmussen, D. M. Shlaes, Mechanism of Inhibition of the Class A beta-Lactamases PCI and TEM-1 by Tazobactam. Observation of Reaction Products by Electrospray Ionization Mass Spectrometry , J. Biol. Chem. 2000, 275, 26674-26682. 

Barak R, Ordentlich A, Barak D, Fischer M, Benschop HP, et al. 1997. Direct determination of the chemical composition of acetylcholinesterase phosphonylation products utilizing electrospray-ionization mass spectrometry. FEES Lett... 

M. A. Strege High-performance liquid chromatographic-electrospray ionization mass spectrometric analyses for the integration of natural products with modem high-... 

Liquid Chromatography-Electrospray Ionization Mass Spectrometric Characteristics ([M - H] and Product Ions) of Selected Proanthocyanidin Tetramers and Pentamers from Pinto Beans, Plums, and Cinnamon, Respectively... 

Liquid chromatography-electrospray ionization mass spectrometry (LC-ESTMS) for analysis of sildenafil has also been developed and applied to 40 botanical products. About half of the analyzed samples proved to contain undeclared additives of the three drugs sildenafil, vardenafil, and tadalafil <2004JPBA525>. A similar electrokinetic capillary chromatography method to that proposed for the determination of sildenafil 82 has been reported for vardenafil 98 and tadalafile 99. Statistical evaluation of the electrophoretic results was achieved. The described method is thought to be rapid and sensitive <2004JCH231>. 

Nozaki, K., Kitagawa, H., Kimura, S., Kagayama, A., and Arakawa, R. (2006). Investigation of the electrochemical oxidation products of zotepine and their fragmentation using on-line electrochemistry/electrospray ionization mass spectrometry. J. Mass Spectrom. 41 606-612. 

Lamouroux, C., Aychet, N., Lelievre, A., Jankowski, C.K., Moulin, C. 2004. High-performance liquid chromatography with electrospray ionization mass spectrometry and diode array detection in the identification and quantification of the degradation products of calix[4]arene crown-6 under radiolysis. Rapid Commun. Mass Spectrom. 18 1493-1503. 

Burdick, D. J. Stults, J. T. 1997. Analysis of peptide synthesis products by electrospray ionization mass spectrometry. Methods Enzymol., 289, 499-519. 

As part of a study to identify the degradation products of sulfur mustard, bis(2-chloroethyl) sulfide, the behavior of various sulfonium salts towards electrospray ionization mass spectrometry has been investigated. Among the compounds studied was 3 -(2-chloroethyl)pentamethylene sulfonium tetrafluoroborate. The ion was readily detected at the 0.01 M level with no ion fragmentation <1997JMP1247>. 

In the authors and several other OPCW-designa-ted laboratories, LC/MS is used as the initial screening procedure for water samples and aqueous extracts of matrices such as soil. This usually provides a tentative identihcation of polar analytes within half a day, on the basis of molecular mass, any fragment ions present, and retention time. A second analysis, under LC/MS/MS conditions, usually provides a firmer identihcation on the basis of a limited number of product ions, most of which result from simple neutral losses. With clean matrices, the initial screening may be performed even faster using how injection or infusion rather than LC (14). An example of the application of how injection with electrospray ionization/mass spectrometry (ESI/MS) in an OPCW prohciency test is provided by Hooijschuur et al. (21) The identihcation of the analytes is usually conhrmed by GC/MS (in most cases after derivatization) as the second technique. 

E.W.J. Hooijschuur, Ch.E. Kientz, A.G. Hulst and U.A.Th. Brinkman, Determination of hydrolysis products of sulfur mustards by reversed-phase microcolumn liquid chromatography coupled online with sulfur flame photometric detection and electrospray ionization mass spectrometry using large-volume injections and peak compression, Anal Chem., 72, 1199-1206 (2000). 

Strege summarized the technique of high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESI-MS) in dereplication of natural products. In contrast to earlier electron impact ionization (El), ESI technique is applicable to virtually any ion in solution with a soft ionization process. A comparison of ESI with fast atom bombardment (FAB), matrix assisted laser desorption ionization (MALDI), atmospheric pressure chemical ionization (APCI) and other techniques demonstrates its superior sensitivity, compatibility and reliability when coupled with HPLC [51]. 

The final product is controlled by electrospray ionization mass spectroscopy. If the mass obtained does not correspond to that predicted for the chemokine with the formation of two disulfide bonds, amino terminal sequencing and amino acid composition analysis is used to identify possible mutations. 

Coupling CE with electrospray ionization mass spectrometry (MS) can potentially be a very powerful tool for detecting and identifying product-related impurities in recombinant pharmaceuticals. Proteins can be detected in the femtomole range with this mode. Conceivably, the bioanalyst could perform a peptide map with CZE-MS and detect, identify, and sequence aberrant peptides derived from degradates. 

Reprinted with permission from Electrospray Ionization Mass Spectrometry Reveals an Unexpected Coupling Product in the Copper- Promoted Synt hesis of Pyrazoles K. Jiang, G. Bian, Y. Chai, H. Yang.Q, Lai and Y. Pan./nt.J. Mass Spectrom. 2012, 321-322,40-48. Copyright 2013, American Chemical Society. 

Ghassempour, A. et al. Monitoring of N-nitrosodiethanolamine in cosmetic products by ion-pair complex liquid chromatography and identification with negative ion electrospray ionization mass spectrometry. J. Chromatogr. A. 2008, 1185,43-48. 

Different techniques have been used to study the products of photoreactions of organometallic compounds for example, irradiation of the arene complexes [CpFe() -arene)]+ resulted in the substitution of the arene by solvent or other potential ligands present in solution. In solutions containing an epoxide monomer, this photochemical reaction generated a species that initiated polymerization. Ion cyclotron resonance Fourier transform mass spectrometry and electrospray ionization mass spectrometry were used to elucidate the mechanism of these photoinitiated polymerizations. 

Ganguly, A.K. Pramanik, B.N. Chen, G. Shipkova, P.A. Characterization of pharmaceuticals and natural products by electrospray ionization mass spectrometry. Pract. Spec-trosc. 2002, 32 (Applied Electrospray Mass Spectrometry), 149-185. 

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