Electrophoretic fingerprint

Rowell and co-workers [62-64] have developed an electrophoretic fingerprint to uniquely characterize the properties of charged colloidal particles. They present contour diagrams of the electrophoretic mobility as a function of the suspension pH and specific conductance, pX. These fingerprints illustrate anomalies and specific characteristics of the charged colloidal surface. A more sophisticated electroacoustic measurement provides the particle size distribution and potential in a polydisperse suspension. Not limited to dilute suspensions, in this experiment, one characterizes the sonic waves generated by the motion of particles in an alternating electric field. O Brien and co-workers have an excellent review of this technique [65]. 

A. Denaturing Gradient Gel Electrophoretic Fingerprints of Microbial Communities... 

Yezek, L. et al.. Adsorption of sodium dodecyl sulfate to colloidal titanium dioxide An electrophoretic fingerprinting investigation, J. Colloid Interf. Sci., T15. 227, 2000. 

Donath E, Walther D, Shilov VN, Knippel E, Budde A, Lowack K, et al. Nonlinear hairy layer theory of electrophoretic fingerprinting applied to consecutive layer by layer polyelectrolyte adsorption onto charged polystyrene latex particles. Lemgmuir 1997 13(20) 5294-305. 

PAH/PSS multilayers deposited on latex particles offer the possibility of electrophoretic mobility measurements. The electrophoretic mobilities were measured as a function of pH and ionic strength (electrophoretic fingerprinting) [105], showing that, for pH values below approximately 8, the surface appears to be positively charged upon PAH adsorption. At higher pH, the negative charge... 

PMF is most effective when the precise sequence of the protein already exists in the protein databases. It works effectively with proteins isolated by either chromatographic or electrophoretic methods, and with a sensitivity that is commensurate with such separation techniques applied to microgram or nanogram quantities of protein. For PMF to work optimally, the protein for which a fingerprint has been obtained must be present in the sequence tabase. A significant limitation in PMF is the difficulty of matching a fingerprint derived from species other than those that are represented in the sequence... 

Wetton, J.H. D.T Parkin 1989 DNA fingerprinting of house sparrows, in Electrophoretic studies on agricultural pests eds H.D.Loxdale J.den Hollander) Syst. Ass. Special Vol., Oxford U.P. 

High-voltage paper electrophoresis was the first method introduced for the identification of dansylated amino acids and for the separation of peptides [1,6] subsequently, electrophoretic separations on thin layers were also reported [34,35]. These methods are, however, unsuitable for the separation of amine derivatives. TLC on silica gel hamino acid, f>eptide and amine derivatives. A large variety of solvent systems of varying polarity is now available, which allows the separation of even complex mixtures of amino acids [7,36—38] and amines [7,17,39]. Fingerprinting or identification of Dns-peptides can also be carried out on silica gel layers 29,40,41]. For the separation of Dns-peptides, electrophoresis in polyacrylamide gel and on cellogel strips was reconunended (42,43]. Alumina [44] kieselguhr (45] and cellulose thin-layers [46] have also been tried. 

AE is readily separated by CZE if it is first derivatized to make it anionic. LAS can be determined in the same chromatogram because LAS has higher electrophoretic mobility and hence a longer migration time to the cathode than derivatized AE. Because of the complexity of commercial AE, CZE has been proposed as a method of fingerprinting products rather than quantitatively determining the isomer/homolog content (18). 

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