Electrophoretic mobility shift analysis EMSA

Abbreviations used EMSA, electrophoretic mobility shift analysis IMPase 1, inositol monophosphatase 1 inositol synthase, A/vo-inositol 3-phosphate synthase NCBI, National Center for Biotechnology Information PCR, polymerase chain reaction Rb, retinoblastoma protein tss, transcriptional start site IP3, Inositol trisphosphate DAG, diacyl glycerol FISH, fluorescent in situ hybridization. [Pg.293]

The electrophoretic mobility shift assay (EMSA), also called the gel-shift or band-shift assay, is more useful than the footprinting assay for quantitative analysis of DNA-binding proteins. In general, the electrophoretic mobility of a DNA fragment is reduced when it is complexed to protein, causing a shift in the location of the fragment band. This assay can be used to detect a transcription factor in protein... [Pg.459]

The abbreviations used are HMG-CoA, 3-hydroxy-3-melhylglutaryl-CoA CAT, chloramphenicol acetyltransferase PPAR, peroxisome proliferator-activated receptor PPRE, peroxisome proliferator-responsive element NRRE, nuclear receptor responsive element RXR, retinoid X receptor hRXRa, human 9-cis-retinoic acid receptor a mPPARa, mouse peroxisome proliferator-activated receptor a COUP-TP, chicken ovalbumin upstream-promoter transcription factor, HNF-4, hepatocyte nuclear factor 4 EMSA, electrophoretic mobility shift analysis tk, thymidine kinase NEFA, nonesterified fatty acids... [Pg.84]