High-performance capillary electrophoretic analysis

Kuo, K.L., Huang, H.Y., and Hsieh, Y.Z., High-performance capillary electrophoretic analysis of synthetic food colorants, Chromatographia, 47, 249, 1998. 

Landers, J.P., Schuchard, M.D., Subramaniam, M., Sismelich, T.R, and Spelsberg, T.C. High-performance capillary electrophoretic analysis of chloramphenicol acetyl transferase activity. J. Chromatogr., 603, 247, 1992. 

Performance and Noise Determination for High-Performance Capillary Electrophoretic (HPCE) Analysis of a Standard Mixture (5 mg/L) of Daidzein and Genistein... 

Fan et al. [106] developed a high performance capillary electrophoresis method for the analysis of primaquine and its trifluoroacetyl derivative. The method is based on the mode of capillary-zone electrophoresis in the Bio-Rad HPE-100 capillary electrophoresis system effects of some factors in the electrophoretic conditions on the separation of primaquine and trifluoroacetyl primaquine were studied. Methyl ephedrine was used as the internal standard and the detection was carried out at 210 nm. A linear relationship was obtained between the ratio of peak area of sample and internal standard and corresponding concentration of sample. The relative standard deviations of migration time and the ratio of peak area of within-day and between-day for replicate injections were <0.6% and 5.0%, respectively. 

High performance capillary electrophoresis is one form of free-solution electrophoresis. CE is useful to researchers and analysts working in areas in which traditional electrophoresis is customarily applied (e.g., biopolymer analysis) and also in disciplines not usually associated with electrophoretic analysis, such as inorganic ion analysis. The potential application areas of CE are vast, because this technique can separate a variety of ligates, from inorganic ions up to intact cells, using the same instrumental hardware designed for separations based on different physical-chemical mechanisms. 

This chapter discussed the concepts and principles of high performance capillary electrophoresis. A brief history of CE was followed by an analysis of the components of an CE system. Two types of separation were presented, electrophoretic migration and electroosmosis. Several types of CE were dis-... 

Ulfelder KJ, Shieh P (1993) Capillary electrophoretic analysis of PCR products using laser-induced fluorescence detection. Poster T-211 presented at the Fifth International Symposium on High Performance Capillary Electrophoresis, Orlando, FL. 

In conclusion, CE is a valuable analytical tool that offers a number of possibilities for the analysis of a wide spectrum of forensicaUy interesting compounds. Practically all compounds which have been traditionally analyzed by GC, high-performance Uquid chromatography, thin-layer chromatography, or slab-gel electrophoresis, can be assayed by capillary electrophoretic procedures. AU methods of capillary electrophoresis can be validated and can meet the demands of good laboratory practice. 

Okafo, G. et al. A coordinated high-performance Uquid chromatographic, capillary electrophoretic, and mass spectrometric approach for the analysis of oligosaccharide mixtures derivatized with 2-aminoacridone. Anal Chem, 68, 4424, 1996. 

Stoeckigt J, Sheludko Y, Unger M, et al. (2002) High-performance liquid chromatography, capillary electrophoretic and capillary electrophoretic-electrospray ionization mass spectrometric analysis of selected alkaloid groups. Journal of Chromatography A 967 85-113. 

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