Proteins electrophoretic analysis

His method was used by Longsworth Machines (Ref 2) for analysis of proteins. Electrophoretic analysis was also used by Svensson (Ref 4) and was described in the book of Weissberger (Ref 5). 

Hiraoka, A., et al (1998). Sodium dodecyl sulfate-capillary gel electrophoretic analysis of molecular mass microheterogeneity of beta-trace protein in cerebrospinal fluid from patients with central nervous system diseases./. Chromatogr. A 802, 143-8. 

Hirose I et al. Proteome analysis of Bacillus subtilis extracellular proteins a two-dimensional protein electrophoretic study. Microbiology 2000 146 65-75. 

WAGONER, W LOSCHKE, D.C., HADWIGER, L.A., Two-dimensional electrophoretic analysis of in vivo and in vitro synthesis of proteins in peas inoculated with compatible and incompatible Fusarium solani, Physiological Plant Path., 1982,20,99-107. 

Results of electrophoretic analysis of proteins in the allantoic fluid and blood serum before and after photodynamic treatment are presented in Figs. 5.5 and 5.6. Visually, we did not detect any changes in the position and intensity of protein bands. In order to quantitatively analyze these parameters we scanned the gels and measured the relative optical density of the bands (Figs. 5.7 and 5.8). 

Righetti, P. G. (2001). Capillary electrophoretic analysis of proteins and peptides of biomedical and pharmacological interest. Biopharm. Drug Dispos. 22, 337—351. 

Traditional sample preparation conditions to form SDS-protein complexes prior to electrophoretic analysis included heat treatment at elevated temperatures (e.g., In the case of non-reduced rMAbs, this could lead to sample preparation artifacts in the form of thermally induced fragmentation attributed to disulfide reduction and exchange reactions. Moreover, it was reported that high pH (>9.0) also enhanced the... 

Although electrophoresis is the method of choice for assessing protein purity, it is not frequently used as a purification step, at least not yet. This is because of the small amounts that can usually be analyzed and because of the denaturing conditions that are often used in electrophoretic analysis. 

Witzmann FA, Fultz CD, Mangipudy RS, Mehendale HM. Two-dimensional electrophoretic analysis of compartment-specific hepatic protein charge modification induced by thioacetamide exposure in rats. Fundam Appl Toxicol 1996 31 124-132. 

Barton et al. developed an assay enabling the study of the influence of DNA-binding proteins simply by gel electrophoretic analysis of oxidative G damage... 

Partridge, M.A.K., Yiang, Y., Skerritt, J.H., and Schaich, K.M. 2003. Immunochemical and electrophoretic analysis of the modification of wheat proteins in extruded flour products. 

Robinson, J. C., B. S. Blumberg, and J. E. Pierce Studies on inherited variants of blood proteins. I. Two dimensional electrophoretic analysis of chromatographic fractions of serum proteins. J. Lab. Clin. Med. 60, 468 (1962). 

One of the first considerations for an electrophoretic analysis of plant proteins is the type of tissue(s) to be used. Virtually any living tissue is suitable (anthers, pollen, flower buds, shoots, roots, megagametophytes, embryos). Specialized protocols for working with pollen leachate (and with organelles) are described by Morden et a/.11 We work primarily with leaves... 

O Keefe, D. O. (1994). Quantitative electrophoretic analysis of proteins labeled with monobromobimane. Anal. Biochem. 222, 86-94. 

Stastny,]., Robertson, A. L, Fosslien, E. (1986). Basic proteins in the human aortic infima nonequilibrium two-dimensional electrophoretic analysis of tissue extracts. Exp. Mol. Pathol. 45, 279-286. 

Mathias, P. I., Fultz, C., Witzmann, F. (1997). Two-dimensional electrophoretic analysis of myocardial proteins from lead-exposed rabbits. Electrophoresis 18, 2978-2982. 

Unlike starch, even in their denatured state, proteins or their dissociated subunits maintain a globular structure. Size reduction by shear is very much less pronounced, so that electrophoretic analysis shows little change in subunit molecular weights between native and extruded materials. 

Molina, E., Ramos M., Martm-Alvarez P. J. (1995). Prediction of the percentages of cows , goats , and ewes milk in Ibeiico cheese by electrophoretic analysis of whey proteins. Z Lebensm. Unters. Forsch., 201, 331-335. 

Moreno-Arribas, M.V., CabeUo, F., Polo, M.C., Martfn-Alvarez, P.J., Pueyo, E. (1999). Assessment of the native electrophoretic analysis of total grape must proteins for the characterization of Vitis vinifera L. culfivars. J. Agric. Food Client., 47, 114-120. 

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