Albumin, electrophoretic analysis separation

This factor together with a dependence of precipitation on the concentration of electrolyte precludes ready quantitative separation of components of different isoelectric point in protein mixtures such as serum (118, 124), Electrophoretic analysis has revealed that one precipitation with SDS achieves only partial separation of the components of a synthetic mixture of purified albumin and globulin buffered in the interisoelectric region (118). 

Analysis of in vivo-formed pellicle by a combination of electrophoretic separation and MALDI-TOF showed the presence of intact histatin 1, cystatin SN, statherin, lysozyme, albumin and amylase [15, 39], In addition, intact cytokeratins 13 and 15 and calgranulin B were identified as components of the salivary pellicle layer for the first time using MALDI-TOF mass spectrometry [15]. Calgranulin B has been shown to be a component of saliva and gingival crevicular fluid [15]. The identification of cytokeratins in the salivary pellicle layer points to the oral epithelium as one of the sources of proteins adsorbed on the tooth surface. 

Lipids circulate in blood as constituents of the lipoproteins or bound to albumin (free fatty acids). Electrophoretic separation of plasma proteins on paper is a relatively easy and convenient method for the semi-quantitative analysis of lipoproteins. Staining the strips with dyes which only respond to lipids reveals three well-defined bands when normal plasma or serum is analyzed. The a-lipoprotein migrates the farthest from the origin. The pre-j8, previously called the a-2-hpoprotein, and the jS-Hpoprotein, are closer to the origin. In the post-prandial normal serum, and in some hyperlipemic sera, after fasting, a fourth band can be seen at the origin. This band is due to neutral fat particles with a small protein content (i.e. chylomicron fraction). 

Fig. 9. Patterns obtained by analysis of fractions taken with a fraction collector following electrophoretic separation in the starch colunm. The upper curve represents an artificial mixture of albumin and y-globulin, the lower curve represents serum. Barbital buffer pH 8.6, T/2 0.1.

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