Electron paramagnetic resonance spectroscopy studies

Electron Paramagnetic Resonance Spectroscopy Studies of Immobilized Monoclonal Antibody Structure and Function... 

Kamoun, D., Merlin, A., Deglise, X., Urizar, S.H., Fernandez, AJM. Electron paramagnetic resonance spectroscopy study of photodegradation of lignins extracted and isolated from Pinus radiata wood. Ann. For. Sci. 56(7), 563-578 (1999)... 

Second law studies by Lucarini, Pedulli, and Cipollone, using electron paramagnetic resonance spectroscopy, have shown that the entropy of this reaction in... 

Based on our current understanding of ribosomal protein synthesis, several strategies have been developed to incorporate amino acids other than the 20 standard proteinogenic amino acids into a peptide using the ribosomal machinery . This allows for the design of peptides with novel properties. On the one hand, such a system can be used to synthesize nonstandard peptides that are important pharmaceuticals. In nature, such peptides are produced by nonribosomal peptide synthetases, which operate in complex pathways. On the other hand, non-natural residues are a useful tool in biochemistry and biophysics to study proteins. For example, incorporation of non-natural residues by the ribosome allows for site-specific labeling of proteins with spin labels for electron paramagnetic resonance spectroscopy, with... 

Electron paramagnetic resonance spectroscopy (EPR) (also called electron spin resonance spectroscopy, ESR) has been scarcely applied in the field of art and art conservation. Some work can be found in which EPR is used as complementary technique to SEM-EDX, NMR, and mass spectrometry (MS) for studying free radicals occurring in polymerization, pyrolytic, oxidative, and other radical degradative processes in artwork, as well as in the characterization of varnishes and oleoresinous media [42]. 

Epimerization of sugar, mechanisms 778 Epimers, definition of 163 Epinephrine (adrenaline) 542,553, 553s Episomes. See plasmid Epithelial cells 29 Epitheliocytes 25 Epoxides, alkyation by 254 Epoxide hydrolases 591 EPR (electron paramagnetic resonance) spectroscopy 398, 399 of glutamate mutase 873 in study of phosphotransferases 639 EPSP (enolpyruvoylshikimate-3-phosphate) 687s... 

Spectroelectrochemical cells that permit redox titrations of precious biological samples, require exclusion of oxygen, and allow acquisition of data from multiple spectroscopic domains have been described. A recent example of these cell designs combines electron paramagnetic resonance spectroscopy with UV-visible absorption spectroscopy [71] for studies of flavoproteins. 

Huttermann J, Ward JF, Myers LS Jr (1971) Electron spin resonance studies of free radicals in irradiated single crystals of 5-methylcytosine. Int J Radiat PhysChem 3 117-129 Huttermann J, Ohlmann J, Schaefer A, Gatzweiler W (1991) The polymorphism of a cytosine anion studied by electron paramagnetic resonance spectroscopy. Int J Radiat Biol 59 1297-1311 Hwang CT, Stumpf CL, Yu Y-Q, Kentamaa HI (1999) Intrinsic acidity and redox properties of the adenine radical cation. Int J Mass Spectrom 182/183 253-259 Ide H, Otsuki N, Nishimoto S, Kagiya T (1985) Photoreduction of thymine glycol sensitized by aromatic amines in aqueous solution. J Chem Soc Perkin Trans 2 1387-1392 Idris Ali KM, Scholes G (1980) Analysis of radiolysis products of aqueous uracil + N20 solutions. J Chem Soc Faraday Trans 176 449-456... 

As noted at the beginning of this chapter, radicals are a unique class of organic intermediates because they can be studied using magnetic resonance techniques such as electron paramagnetic resonance spectroscopy (EPR). The EPR technique has proven valuable for ascertaining the local structure about a radical in a supercritical fluid solvent. 

Due to the complexity of DOM fractionation has revealed more detailed information on the structural subunits prior to the application of advanced analytical methods. Most effective is the combination of different spectroscopic methods using UV-vis absorbance, fluorescence, 1H- and 13C-nuclear magnetic resonance, and Fourier transform-infrared (FT-IR) spectroscopy. In some studies, also electron paramagnetic resonance spectroscopy (EPR) is used (e.g., Chen et al., 2002). 

Studying RNA Using Site-Directed Spin-Labeling and Continuous-Wave Electron Paramagnetic Resonance Spectroscopy... 

Kisseleva, N., Khvorova, A., Westhof, E., and Schiemann, O. (2005). Binding of manganese (II) to a tertiary stabilized hammerhead ribozyme as studied by electron paramagnetic resonance spectroscopy. RNA 11, 1—6. 

A purely organic chiral nitroxide which shows liquid crystalline behaviour as well as intriguing magnetic properties and a dependence on the enantiomeric nature has been reported [180]. The reason for studying the compounds was to increase the sensitivity of mesophases to magnetic and electric fields. The racemic modification of the radical, which displays a nematic phase, proved to be more sensitive to alignment than the cholesteric phase with the enantiomers present. It was proposed that the compounds may also be used to study the dynamic nature of mesophases by electron paramagnetic resonance spectroscopy. 

Ballou DP, Palmer GA. Practical rapid quenching instrument for study of reaction-mechanisms by electron-paramagnetic resonance spectroscopy. Anal Chem. 1974 46 1248-53. 

Electron paramagnetic resonance spectroscopy has contributed greatly to the understanding of the configuration of the iron in LAM. In initial spectroscopic studies, the as-purified enzyme exhibited a rhombic spectrum at 10 K. The signal was centered at g = 2.007, and underwent power saturation at the same temperature in a homogeneous manner with a = 15 2 mW. At higher temperatures the... 

CapeillEre-Blandin, C., Bray, R. C., Iwatsubo, M., and Labeyrie, F., 1975, Flavocytochrome 2 Kinetic studies by absorbance and electron paramagnetic resonance spectroscopy of electron distribution among prosthetic groups, Eur. J. Biochem. 54 549n566. 

Electron paramagnetic resonance spectroscopy has proved a valuable tool in the study of AdoCbl-dependent enzymes. AdoCbl itself is EPR-silent, but upon homolysis to form Cbl(II), two spins are formed, one on the cobalt (which now has low-spin d configuration) and one on the organic radical. Typically, the two unpaired electrons remain close enough in the enzymeis active site that they interact with one another to give complex, but informative, EPR spectra. 

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