Electrochemical Nucleic Acid Biosensors

Electrochemical nucleic acid biosensors are based on electrochemical transduction of the hybridization event and show great promise for detection of specific gene sequences related to inherited and infectious diseases. Electrochemical detection of specific DNA sequences has an advantage in reducing the size of the total detection system [36], The advantages of electrochemical nucleic acid biosensors include potential of miniaturization, short response time, ease of use, low cost, and compatibility with microfabrication techniques [37], 

Electrochemical DNA biosensors are divided into two main groups  

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J. Wang, Electrochemical nucleic acid biosensors. Anal. Chim. Acta 469, 63—71 (2002). 

Wang, J., 2002. Electrochemical nucleic acid biosensors. Anal. Chim. Acta, 469, pp. 63-71. Wang, J., Cai, X.H., Rivas, G., Shiraishi, H., Farias, P.A.M., and Dontha, N., 1996. DNA electrochemical biosensor for the detection of short DNA sequences related to the human immunodeficiency virus. Anal. Chem., 68, pp. 2629-2634. 

Electrochemical Nucleic Acid Biosensors Based on Hybridization Detection for Clinical Analysis... 

Label-Based Electrochemical Nucleic Acid Biosensors... 

Early studies on electrochemical nucleic acid biosensors were based on electrochemical transduction of redox labels (indicators) that have significant different behaviors between dsDNA and ssDNA. These intercalator molecules have higher binding affinity to dsDNA than ssDNA. Mikkelsen and coworkers investigated this approach by using Co(phen) as a hybridization indicator. The intercalator molecule was accumulated at ss and dsDNA at covalently attached... 

The aim of this book is to cover the full scope of electrochemical nucleic acid biosensors by emphazing on DNA detection. The material is presented in 16 chapters. Starting with the terminology related to electrochemical DNA-based biosensors in Chapter 1, the researchers active in the fields of biosensor design, molecular biology, and genetics describe types of detection used for analysis (chapters 6, 9, 11, and 13), types of materials used for biosensor design (chapters 3, 4, 5, 8, 10, and 14), and types of nucleic acid interactions detected (chapters 2, 7,12, and 15). 

Prabhakar N, Sumana G, Arora K, Singh H, Malholra BD (2008) Improved electrochemical nucleic acid biosensor based on polyaniline-polyvinyl sulphonate. Electrochim Acta 53 4344-4350... 

Wang J (2002) Electrochemical nucleic acid biosensors. Trends Anal Chtan 21 226-232... 

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