Enhanced green fluorescent protein EGFP

EGFP Enhanced green fluorescent protein Panning on plastic-immobilized protein... 

EBFP Enhanced Blue Fluorescent Protein ECFP Enhanced Cyan Fluorescent Protein EGFP Enhanced Green Fluorescent Protein EYFP Enhanced Yellow Fluorescent Protein. 

RhoB [also enhanced green fluorescent protein (EGFP)-RhoB, see above]... 

DOTMA E. coli E EBV ECFP ECV EGFP ELISA EYFP FACS FdG FH2 FH4 FK506 FLP propane-aminium-trifluoracetate 7V-[2,3-(dioleyloxy) propyl]-/V,/V,/V-trimethyl ammonium chloride Escherichia coli erythromycine operon/repressor Epstein-Barr virus enhanced cyan fluorescence protein extracellular viral particles enhanced green fluorescence protein enzyme-linked immunosorbent assay enhanced yellow fluorescence protein fluorescence-activated cell sorter fluorescein di- 3-D-galactopyranoside dihydrofolate tetrahydrofolate human immunophilins native recombinase isolated from the 2pm plasmid from Saccharomyces cerevisiae... 

Also, the Phe-64 -> Leu and Ser-65 —> Thr mutant shows higher fluorescence parameters than that of the wild GFP. Excitation at 458 nm yields a fluorescence spectrum with two peaks at 512 and 530 nm. The fluorescence properties of this enhanced green fluorescent protein (EGFP) were found to be similar to the recombinant glutathione S-transferase-EGFP (GST-EGFP) protein, expressed in Escherichia coli (Cinelli etal. 2004). 

Different-sized plasmid DNA may be used to create these polymer/DNA particles. For easy visual inspection of gene expression and quantitative cell population information enhanced green fluorescent protein (EGFP) DNA is often used. For high-throughput screening applications, luciferase (Luc) DNA is often used instead. 

Fig. 5. Electronic spectra of a mixture of ferric cytochrome c and enhanced green fluorescent protein EGFP before (—) and after (—) 490 nm photolysis indicating the formation of the ferrous form of heme c (6) with maxima at 413, 520, and 550 nm. Adapted from Ref (37).

Regional lymph nodes were removed 36 h after injection. The flow cytometric analysis of the crude lymph node cell suspension showed that 1.2% of the cells expressed the enhanced green fluorescent protein (EGFP) in comparison to cells from untreated controls (Fig. 4a). 

EGFP (biotinylated) Biotinylated Enhanced green fluorescent protein Panning in solution followed by capture on streptavidin-magnetic beads... 

The three-dimensional network of actin stress fiber, which is an association of actin filaments, provides mechanical support for the cell, determines the cell shape, and enables cell movement. Thus the shape change in the cell due to the laser tsunami can be examined by observing the laser-induced dynamics of fibers. The actin stress fiber was visualized by binding it with enhanced green fluorescence protein (EGFP), and monitored by total internal reflection fluorescence (TIRF) imaging [34]. 

Ahmed AH, Samuel TH, Watt WW. Multiphoton molecular spectroscopy and excited-state dynamics of enhanced green fluorescent protein (EGFP) acid-base specificity. Chem Phys 2001 274 37-55. 

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