Effect of incubation temperature

Wu Q, DL Bedard, J Wiegel (1997a) Effect of incubation temperature on the route of microbial reductive dechlorination of 2,3,4,6-tetrachlorobiphenyl in polychlorinated biphenyl (PCB)-contaminated and PCB-free freshwater sediments. Appl Environ Microbiol 63 2836-2843. 

Figure 2 (A) Effect of incubation temperature on uptake of doxorubicin into 200 nm EPC/cholesterol (55 45 mol/mol) large unilamellar vesicles (LUVs) exhibiting a transmembrane pH gradient (pH 4 inside, 7.8 outside). Doxorubicin was added to LUVs (D/L = 0.3 wt wt) equilibrated at 21°C, 37°C, and 60°C. (B) Effect of cholesterol on the uptake of doxorubicin at 20 into lOOnm LUVs exhibiting a transmembrane pH gradient (pH 4.6 inside, 7.5 outside). Lipid compositions were EPC and EPC/cholesterol (1 1 mol/mol). The initial drug-to-lipid ratio was 100 nmol/pmol. Source Prom Refs. 12 (A), 21 (B).

Response to prey chemical cues by hatchling pine snakes (Pittwphis melanoleucus). Effects of incubation temperature and experience. Journal of Chemical Ecology 17, 1069-1078. 

Figure 1. Effect of incubation temperature on histamine formation in skipjack tuna.

The effect of incubation temperature, initial cake moisture, and olive oil supplementation on lipase production was evaluated following a two-level experimental plan. The range of study of the variables was chosen based on previous results of the our group (2,7,10). 

Figure 3 Effect of incubation temperature on specific binding of [3H]Pr-BP. Membranes (1 mg protein) were incubated with 1.7 nM [ H]Pr-BP in 1 ml of 50 mM Tris-citrate buffer (pH 7.4 at 3°C) for 1 h at various temperatures. After termination of the reaction, filters were rinsed with the buffer maintained at the incubation temperature. Each point represents the mean SD of three experiments. Reproduced with permission from Ref. 39. Copyright 1986, Academic Press Inc.

Westerman, P., The effect of incubation temperature on steady-state concentrations of hydrogen and volatile fatty acids during anaerobic degradation in slurries from wetland sediments, FEMS Microbiol. EcoL, 13, 295-302, 1994. 

FIGURE 12.5 Effect of incubation temperature on cell mass ( ), lipid content ( ), rate of A6-desaturation of linoleic acid to y-linolenic acid ( ), and percentage of y-linolenic acid in total lipids ([]) in the transformed Y. lipolytica. A6-Desaturation was calculated as [Product]/ [Product + Substrate] x 100%. Each value point represents the mean of three independent experiments. In each category, values with different letters or numbers are significantly different from each other at p <. 05. 

Effect of Incubation Temperature on CDs Formation The Incubation pH was adjusted to pH 6.0 with 100 mM Macllvaine buffer and other incubation conditions were the same as the previous experiment. The results are shown in Fig. 4. The enzyme reaction proceeded best in the range of 40 C to 70"C for the CGTase from B. ohbensls. In the enzyme from B. circulans, the reaction proceeded best from 40 C to 80 C. But the CGTase from B. macerans had a very narrow temperature activity range around 50 C. 

Gott, C. L., and R. P. Williams Effect of incubation temperature and nutrition upon pigmentation in Serratia marcescens (Abstract). Texas Repts Biol. Med. 18, 360 (I960). 

Figure 3 shows the effects of incubating surfactin from 21 to 70°C. Although there was an apparent decrease in surface tension of surfactin at higher temperature, there was also a decrease in surface tension of water as... 

Figure 7.11. The effects of exposure temperature on protein synthetic patterns of isolated gill tissue from specimens of 13°C-acclimated Tegula funebralis. Autoradiographic images illustrate newly synthesized (35S-labeled) proteins of several size classes (molecular mass standards are shown in the left lane). Two specimens from each temperature of incubation are shown. At temperatures above 24°C, synthesis of heat-shock proteins in the molecular mass ranges of 38, 70, 77, and 90 kDa is induced. Hsp synthesis becomes an increasingly large fraction of protein synthesis as exposure temperature increases, and by 38°C, only synthesis of hsp70 is observed. By 39° C, no protein synthesis takes place. (Figure modified after Tomanek and Somero, 1999.)...

Haq et al.P l presented the time evolution of diamond nucleation on Si(lOO) in MW PACVD, as shown in Fig, 1. The effect of substrate temperature on the nucleation processes is evident in the figure. For each substrate temperature, the nucleation density increases drastically after the incubation period, and then rapidly attains its saturation level. For substrate temperatures less than 816°C, higher substrate temperatures lead to shorter incubation periods, higher nucleation rates and higher nucleation densities. 

Lehtimaki, J. et al.. The effects of incubation time, temperature, light, sahnity, and phosphorous on growth and hepatotoxin production by Nodularia strains. Arch. Hydrobiol, 130, 269, 1994. 

Roy et al. (1996) studied the effect of incubation time and temperature on the production of antifungal compounds by Lactococcus lactis subsp. lactis, and they reported an optimum production at 30 °C after 48 h of incubation. Corsetti et al. (1998) also reported maximum production of organic acids, with antifungal activity, by L. sanfrancisco (L sanfranciscensis) after 48 h of incubation at 30 °C, when the initial pH of the medium was 6.0. 

Fig. 14 Optimization of hybridization conditions, (A) Plot of peak current response vs. incubation time (t from 10 min to 40 min), (B) Effect of hybridization temperature (from 40°Cto 70 °C), The differential pulse voltammetry peak current of CA-MB was detected in 0,1 M phosphate buffer saline solution pH 7,3, Reproduced from Li with permission... 

Andersen et al. (1996) and Andersen (1995) have studied the effect of temperature on the recombinant protein production using a baulovinis/insect cell expression system. In Tables 17.15, 17.16, 17.17, 17.18 and 17.19 we reproduce the growth data obtained in spinner flasks (batch cultures) using Bombyx mori (Bm5) cells adapted to serum-free media (Ex-Cell 400). The working volume was 125 ml and samples were taken twice daily. The cultures were carried out at six different incubation temperatures (22, 26,28, 30 and 32 TT). 

---