Drug binding studies

Other mitogenicity in CHO-K1 and human BIO osteosarcoma cells, human hepatoma cells and MCF-7 cells to include drug binding study to IGF-1 and insulin receptors local tolerance in pigs immunogenicity studies in rabbits... 

It is well known that drugs bind to plasma proteins, particularly to serum albumin and a-acid glycoprotein, and that only the unbound, or free, fraction is responsible for any pharmacological effect. For protein-drug binding studies size-exclusion chromatography in one of three variants—namely, the Hum-mel-Dreyer method (1962), the vacancy peak method (Sebille, et al., 1979), and frontal analysis (Cooper and Wood, 1968)—is the traditional method of... 

These preliminary studies culminated in a wealth of activity at SR sources devoted to virus crystal data collection both for structure solving and also drug binding-studies. Table 10.10 gathers the various references to this type of work up to 1990. We now give further details on the rhinovirus work which was done primarily on CHESS after the initial work at Hamburg and Daresbury. This case study will then be followed by a description of the FMDV work done at Daresbury using the SRS. 

R. Maleki, A.A. Matin, R. Hosseinzadeh, A. Jouyban, PVC membrane sensor for diclofenac Applications in pharmaceutical analysis and drug binding studies, Pharmazie, 62 (9), 672-677, 2007. 

For a review, see M. Casely-Hayford, M. Searcey, The azinomycins. Discovery, synthesis and DNA-binding studies. in DNA and RNA hinders. From small molecules to drugs, Wiley-VCH, Weinheim, 2002. pp. 676 696. 

Han Nl, Lee YS, Choi H, Choi JY, Yun SK, Cho SH, Han JY, Yang JM, Ahn BM, Choi SW, Lee CD, Cha SB, Sun HS, Park DH (2002) PCNA expression and electron microscopic study of acinus-forming hepatocytes in chronic hepatitis B. Korean J Intern Med 17 100-106 Herve F, Urien S, Albengres E, Duche JC, TiUement IP (1994) Drug binding in plasma. A summary of recent trends in the study of drug and hormone binding. Qin Pharmacokinet 26 44-58... 

Westman, J. Boulanger, Y. Ehrenberg, A. Smith, I. C. P., Charge and pH dependent drug binding to model membranes A 2H-NMR and light absorption study, Biochim. Biophys. Acta 685, 315-328 (1982). 

Compared with the conventions adopted for discussing the relationship between drug concentration and response (Chapter 1), a rather different terminology has evolved for ligand-binding studies. 

Lebel, L. A. Koe, B. K. (1992). Binding studies with the 5-HTlb receptor agonist [3H]CP-96,501 in brain tissues. Drug Devel. Res. 27, 253-64. 

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