Double mutant cycles

Serrano L, Bycroft M, Fersht AR. Aromatic-aromatic interactions and protein stability. Investigation of double mutant cycles. J Mol Biol 1990 218 465-475. 

Pons, J. Rajpal, A. Kirsch, J.F., Energetic analysis of an antibody/antigen interface alanine scanning mutagenesis and double mutant cycles on the HyHEL-10/lysozyme interaction, Prot. Sci. 1999, 8, 958-968. 

Horovitz, A., Double-mutant cycles a powerful tool for analyzing protein structure and function, Fold. Des. 1996,1, R121-126. 

Roisman LC, Piehler J, Trosset JY et al (2001) Structure of the interferon-receptor complex determined by distance constraints from double-mutant cycles and flexible docking. Proc Natl Acad Sci U S A 98 13231-13236... 

Double mutant cycles—see thermodynamic cycles Double-sieve editing mechanisms 387-389, 398, 399... 

T4 lysozyme 33,497 helix stability of 528, 529 hydrophobic core stability of 533, 544 Tanford j8 value 544, 555, 578, 582-Temperature jump 137, 138, 541 protein folding 593 Terminal transferase 408,410 Ternary complex 120 Tertiary structure 22 Theorell-Chance mechanism 120 Thermodynamic cycles 125-131 acid denaturation 516,517 alchemical steps 129 double mutant cycles 129-131, 594 mutant cycles 129 specificity 381, 383 Thermolysin 22, 30,483-486 Thiamine pyrophosphate 62, 83 - 84 Thionesters 478 Thiol proteases 473,482 TNfn3 domain O-value analysis 594 folding kinetics 552 Torsion angle 16-18 Tbs-L-phenylalanine chloromethyl ketone (TPCK) 278, 475 Transaldolase 79 Tyransducin-o 315-317 Transit time 123-125 Transition state 47-49 definition 55... 

Ambiguity in partner Not for double mutant cycles Yes, unless a... 

Double Mutant Cycles - Quantifying Weak Interactions... 

S"- Cockroft, S. L. and Hunter, C. A., Chemical double-mutant cycles dissecting non-covalent interactions , Chem. Soc. Rev. 2007, 36, 172-188. 

Figure 10.20 A chemical double mutant cycle to assess the strength of edge-to-face n-n interactions (thick dashed wedges) in a molecular zipper complex AAG = AGA — ACB — AGc + A )>...

Interactions Analysis of the Barnase-Barstar Interface by Single Mutations and Double Mutant Cycles. 

Schreiber G, Fersht AR (1995) Energetics of protein-protein interactions analysis of the barnase-barstar interface by single mutations and double mutant cycles, J Mol Biol, 248 -178 1X9... 

Cavanagh, J., Venters, R.A. (2001) A structural double-mutant cycle estimating the strength of a buried salt bridge in barnase. Protein dynamic studies move to a new time slot, Nat. Struct. Biol. 8 (11), 912-914. 

Luisi DL et al (2003) Surface salt bridges, double-mutant cycles, and protein stability an experimental and computational analysis of the interaction of the Asp 23 side chain with the N-terminus of the N-terminal domain of the ribosomal protein 19. Biochemistry... 

The reasons for the observed differences between actual and expected coupling energies for most of the hydrogen-bonded residue pairs we examined can be understood in terms of an analysis of the theory of double-mutant cycles by Serrano et al. (1990). If A and B are two hydrogen-bonded residues and the mutations A A and B B are... 

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