Double labeling systems

Incorrect Label materials, lines, pumps and valves chemicals used.. Check labels against batch sheets Set valves to correct flow path Use double check system Use procedures and training Use different packaging for different chemicals API RP 750 CCPS G-3 CCPS G-22 CCPS G-30 CCPS G-33... 

To characterize phospholipid oxidation during apoptosis, cellular phospholipids were metabolically labeled at sn-2 position with a natural unsaturated florescent fatty acid containing four conjugated double bonds, cw-parinaric acid (cw-PnA) (Kagan et al, 1998 Tyurina et al, 2001). Oxidative desttuction of any part of the conjugated double bond system of... 

Finally, an ABC assay system is nice to have available for detecting more than one antibody on an individual specimen. In double-labeling experiments, having two completely different assay systems reduces the chances of crossover reactivity. The first antigen can be detected with the standard ABC procedure, and the second antigen is then detected using the PAP system (see... 

Reaction of Atomic Carbon with Pyrroie. Cocondensation of arc generated C with pyrrole 71 gives pyridine 72. This clean reaction is useful to insure that the system is functioning properly when setting up a new carbon arc reactor. Double labeling experiments with atoms and produce 72 with both the... 

Structural Elucidation of the Stable Intermediate for the SSI-Subtilisin System by 13C NMR Spectroscopy The other benefit of the l5N, l3C-double labeling, which may in fact be more crucial for... 

The importance of proper instrument and experimental standards to evaluate the machine performance and experimental variables should not be underestimated. We recommend to check for even illumination, PSF, system/laser stability and correct deviations, when necessary, using proper procedures such as fluorescence slides and triple or double-labeled fluorescence beads containing defined amounts of fluorophores. 

In order to pursue heteronuclear multidimensional NMR experiments, a bacterial system for expression of apoLp-III has been developed which allows facile production of 150 - 200 mg/L l N-iabeled apoLp-III or 100 - 125 mg/L 15N/l3c-double labeled apoLp-III. Figure 2, panel A shows the IH- n HSQC spectrum of a 1.0 mM solution of lipid-free, uniformly N-labeled apoLp-III. Panel A also indicates that, although the chemical shift dispersion in the H-dimension is rather small (6.5 ppm to 9.5 ppm), it is generally upfield shifted, consistent with the fact that the protein secondary structure is predominantly a-helix (13). The chemical shifts in the N-dimension are well-dispersed which results in good separation of the overall crosspeaks. However, certain regions in the spectrum are still crowded as shown in Figure 2. 

Table 23.1. Euler angles of the C and N CSA principle axis system (PAS) relative to fiber axis system (FAS) for [l- C]Gly, [l- C]Ala, [ N]Gly and [ NjAla sites of B. mori silk fibroin samples and that relative to molecular symmetry axis (MSA) for [l- C]-[ N]double labeled sites of Boc-Gly-Ala- [l- C]Gly-[ N]Ala-Gly-Ala-OPac and Boc-Ala-Gly-[l- C]Ala-[ N]Gly-Ala-Gly-OPac...

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