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DNA organization

Inactive and active chromatin domains can be defined at the molecular level by the presence (or absence) of specific histone modifications (or combinations of modifications), by the degree of chromatin compaction and the presence of chromatin associated proteins. Several earlier studies focusing on specific genes or loci showed that active chromatin is generally more accessible and enriched in acetylated forms of histones H3, H4, H2A [5] and histone H3 methylated at lysine 4 (H3/K4) [6]. H3/K4 di- and tri-methylation and H 3 acetylation correlate globally with open chromatin [7]. [Pg.139]

In contrast to this, inactive chromatin is characterized by histone hypoacetylation and the enrichment of histone H3 lysine 9 (H3/K9) methylation [6]. [Pg.140]

Chromatin Immunoprecipitation as a Powerful Method to Study Chromatin [Pg.140]


Not all data favored stepwise linear deletion as the basis for switching. Obata et al. (1981) found that the DNA organization in a particular plasmacytoma could be explained by unequal sister chromatid exchange, but observations inconsistent with this possibility were reported by Wabl et al. (1985). Alt et al. (1982) observed that a subclone of a transformed pre-B cell line had switched from synthesis of a p chain to synthesis of a y chain, with retention of the same VH and JH as well as both copies of the gene these results suggested that the switch in heavy chain synthesis was a transcriptionally controlled event (see discussion below). [Pg.61]

GM organism/genetically modified organism—the term used interchangeably to recombinant-DNA organism see Recombinant-DNA organism. [Pg.401]

Recombinant-DNA organism—an organism in which the genetic material has been changed through in vitro nucleic acid techniques, including recombinant deoxyribonucleic acid (DNA) and recombinant ribonucleic acid (RNA) and direct injection of nucleic acid into cells and organelles. [Pg.403]

Hud, N. V. (1995). Double-stranded DNA organization in bacteriophage heads An alternative toroid-based model. Biophys. J. 69, 1355-1362. [Pg.253]

Gierlich J, Burley P, Gramhch ME, Hammond DM, Carell T. Click chemistry as a reliable method for the high-density postsynthetic functionalization of alkyne-modified DNA. Organic Letters 2006 8 3639-3642. [Pg.547]

RMtrictiun enzyme Cleavage reaclim in DNA Organism used as source of enzyme... [Pg.949]

Figure 2 Schematic presentation of the proviral DNA organization of retroviruses. Figure 2 Schematic presentation of the proviral DNA organization of retroviruses.
D. Animals with brains - the development of the nervous system with a brain allowed the animal to be informed about the environment and to remember experiences. The fast responses were increasingly independent of reference to DNA. Organization in groups seen in patterns of behavior developed. [Pg.459]

OrganizationforEconomicCo-operationsandDevelopment Recommendation of the Council - Concerning Safety Considerations for Applications of Recombinant DNA Organisms in Industry, Agriculture and Envirorunent, OECD, Paris (1986)... [Pg.24]

Acute exposure of large doses may cause radiation poisoning or radiation burns. Chronic exposure may result in cancer or mutations in one s children due damage to DNA. Organs with rapidly dividing cells such as bone marrow, intestines, and gonads are most vulnerable. Some effects do not appear until several years have passed. [Pg.111]


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