Oligonucleotide probe arrays

FIGURE 11.2 Photolithographic synthesis of oligonucleotide probe arrays. 

Soriano MA, Tessier M, Certa U, Gill R. 2000. Parallel gene expression monitoring using oligonucleotide probe arrays of multiple transcripts with an animal model of focal ischemia. J Gereb Blood Flow Metab 20 1045. 

As the density of information derived from efforts to sequence, map and identify human genes increased, so did the demand for analytical tools capable of exploiting this information. DNA microarrays were developed in response to this demand. Southern(69) was the first to describe parallel, in situ ohgonucleotide synthesis as a means of generating oligonucleotide probe arrays on solid supports for highly parallel hybridization analysis. Southern s method uses standard nucleotide synthetic reactions to synthesize the oligonucleotides. The reactions are carried out in a movable chamber, which provides a physical barrier between the reaction chamber and the intended synthesis area. 

Lipshutz RJ, Morris D, Chee M, Hubbell E, Kozal MJ, Shah N, Shen N, Yang R, Fodor SP. Using oligonucleotide probe arrays to access genetic diversity. Bio techniques 1995 19(3) 442—7. Review. 

High-Density GeneChip Oligonucleotide Probe Arrays... 

Keywords GeneChip array, Oligonucleotide probe array. Photolithography, Gene expression monitoring, Genotyping... 

G. H. McGall F. C. Christians High-D n ity GeneOiip Oligonucleotide Probe Arrays... 

Lipshutz RJ et al. Using oligonucleotide probe arrays to access genetic diversity. Bio-techniques 1995 19 442-447. 

Lipshutz, R. J., D. Morris, M. Ghee, E. Hubbell, M. J. Kozal, N. Shah, N. Shen, R. Yang, and S. R Fodor. 1995. Using oligonucleotide probe arrays to access genetic diversity. Biotechniques 19 442-7. 

McGall, G.H., Christians, P.C. High-Density GeneChip Oligonucleotide Probe Arrays. Vol. 77,... 

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