DNA electrophoresis

Chiari, M D Alesio, L Consonni, R Righetti, PG, New Types of Large-Pore Polyacrylamide-Agarose Mixed-Bed Matrices for DNA Electrophoresis Pore Size Estimation from Ferguson Plots of DNA Eragments, Electrophoresis 16, 1337, 1995. 

Slater, GW Rousseau, J Noolandi, J Tunnel, C Lalande, M, Quantitative Analysis of the Three Regimes of DNA Electrophoresis in Agarose Gels, Biopolymers 27, 509, 1988. 

VoUcmuth, WD Austin, RH, DNA Electrophoresis in Microhthographic Arrays, Natnre 358, 600, 1992. 

Bocek, P. and Chrambach, A., Electrophoretic size separations in liquidified agarose of polystyrene particles and circular DNA, Electrophoresis, 12, 620, 1991. 

Cann, J.R., Models of mobility-shift assay of complexes between dimerizing protein and DNA, Electrophoresis, 18, 1092, 1997. 

Gasser, R.B., Zhu, X.Q. and Woods, W.G. (1999) Genotyping Taenia tapeworms by single-strand conformation polymorphism of mitochondrial DNA. Electrophoresis 20, 2834—2837. 

Fig. 9.8 Apoptosis of HEK293 cells induced by SWCNTs. Bl DNA electrophoresis of cells cultured with 25g/ml SWCNTs for 1-5 days, M molecular marker, no. 1-5 denote the results of cells cultured for day 1-5, respectively B2 DNA electrophoresis results of control cells cultured for day 1-5 C the cell cycle distribution of HEK293 cells cultured with 25 lg/ml SWCNTs for 4 days, the percentage of sub-Gl cells (apoptosis cells) was 43.5%. (Grunlan et al., 2000. With permission from Elsevier) (See Color Plates)...

Aparose el for DNA electrophoresis. Agarose is suspended in TAB buffer (40 mM Tris-acetate [pH 8.3], 1 mM EDTA) and dissolved by heating in a microwave oven. When the temperature drops to around 65°C, pour it into trays and allow for gelation. 

Dorfman, K.D., Brenner, H., Modeling DNA electrophoresis in microfluidic entropic trapping devices. Biomed. Microdevices 2002, 4(3), 237-244. 

Agarose. 5. Ethidium Bromide. 6. DNA electrophoresis system (Owl electrophoresis systems, Fisher Scientific). 

Kenward M, McCormick LC> Tessier F. Theory of DNA electrophoresis (approximately 1999-2002). Electrophoresis 2002 23 3791-816. 

D. et al. (2002) Mechanisms of in vivo DNA eiectrotransfer respective contributions of cell electropermeabilization and DNA electrophoresis. Molecular Therapy, 5, 133-140. 

Details of DNA electrophoresis methods are given in Table 9.3. The most widely used buffers in gel electrophoresis of nucleic acids are Tris/acetate/EDTA (TAE) and Tris/borate/EDTA (TBE). TBE has the best buffering capacity but the use of TAE tends to result in somewhat sharper bands. For some purposes, such as DNA extraction with glassmilk, TBE should be avoided unless sorbitol is... 

More recently, electronic eqmpment used in other areas of science has been used in TLC for the evaluation of chromatograms. This includes the flat-bed scanner, which is often used in conjunction with special software for the evaluation of DNA electrophoresis results. However, the direct use of flat-bed scanners in TLC is limited to the evaluation of colored chromatograms, and the author is not aware that any work on this theme has yet been published. 

Documentations obtained when evaluation is performed with a flat-bed scanner are always dependent on the software used and also, of course, on the scanned object. As mentioned in Section 7.4, a flat-bed scanner can be directly used for processing colored chromatograms only. The first investigations were done with the Pharmacia system, which was also used for the evaluation of DNA electrophoresis gels. 

DNA electrophoresis results. A gel after DNA electrophoresis. Lane M is molecular weight markers. The numbers along the left-hand side are the sizes, in bp. at the marker molecule. 

Two-Dimensional Pulsed Field Electrophoresis Theoretical Background. Applying the polymer reptation model to conventional DNA electrophoresis is proving to be quite successful, especially in the case of field strength dependent electrophoretic mobilities. 

Which of the following statements correctly describe a feature of DNA electrophoresis ... 

FIGURE 26.2 Effect of swainsonine on the growth of HuH-6KK treated with S75II. (A) The cells were primed with ( ) or without (O) swainsonine (5 pg/mL) for 6 days, then treated with different concentrations of S75II for 48 h. Cell viability was detected by MTT assay. (B) DNA electrophoresis of HuH-6KK. The cells were primed with or without swainsonine (5 pg/mL) for 6 days, then treated with or without S75II (5 pg/mL). 

Gel electrophoresis is a method for analyzing DNA. Electrophoresis separates DNA or protein by size or electrical charge. The DNA runs towards the positive charge as it separates the DNA fragments by size. The gel is treated with a DNA-binding dye that fluoresces under ultraviolet light. A picture of the gel can be taken and used for analysis. 

Viovy, J.L. and Duke, T., DNA electrophoresis in polymer solutions Ogston sieving, reptation and constraint release. Electrophoresis, 14, 322, 1993. 

Thomas, G., et al., Capillary and microelectrophoretic separations of ligase detection reaction products produced from low-abundant point mutations in genomic DNA, Electrophoresis, 25,1668, 2004. BraziU, S.A. and Kuhr, W.G., A single base extension technique for the analysis of known mutations utilizing capillary gel electrophoresis with electrochemical detection. Anal Chem, 74, 3421, 2002. 

Most DNA sequencing today is done essentially by the same enzymatic method conceived by Frederick Sanger in the 1970s—while DNA electrophoresis instruments and molecular labels have improved (we now use fluorescent dyes, whereas Sanger used radioactive labeling), the basic... 

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